Western blot abreview for Anti-KIF3A antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
200000 cells
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 3% milk in TBST

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG (whole molecule)-peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL plus
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: approximately 95 kDa

Abcam user community

Verified customer

Submitted Oct 13 2008

Sign up