Western blot abreview for Anti-KIF3A antibody

Average
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - other (brain lysate differentially centrifuged, P3)
Loading amount
5 µg
Specification
brain lysate differentially centrifuged, P3
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Donkey serum in TBS-Tween

Secondary antibody

Name
Non-Abcam antibody was used: Donkey Anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000

Detection

Detection method
Millipore Immobilon Western
Exposure
10 second(s)
Bands
Specific: 85 kDa
Positive control
Rat brain lysate, P3 pellet of differential centrifugation

Additional data

Additional Notes
The band is specific and looks good, but unfortunately the background signal is very high even though various blocking agents have been tested.

Abcam user community

Verified customer

Submitted May 24 2011

Sign up