Western blot abreview for Anti-KIF3A antibody

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Abreviews
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS cells)
Loading amount
100 µg
Specification
COS cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBS-T (0.1%)

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 85 kDa
Positive control
COS total cell lysate

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Submitted May 14 2013

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