Immunocytochemistry/ Immunofluorescence abreview for Anti-KIF3A antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH-3T3 cells)
Permeabilization
No
Specification
NIH-3T3 cells
Blocking step
1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 1% Donkey Serum + 1% BSA + 0.1% Triton X
Dilution
1/500

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rabbit Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
NIH-3T3 cells were serum starved for 24 hours prior to staining. Kif3a staining appears punctate cytoplasmic and possibly enriched in primary cilia.

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Submitted Nov 02 2015

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