Anti-KIF3C antibody (ab236748)
Key features and details
- Rabbit polyclonal to KIF3C
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF3C antibody -
Description
Rabbit polyclonal to KIF3C -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan -
Immunogen
Recombinant fragment corresponding to Human KIF3C aa 150-300.
Database link: O14782 -
Positive control
- WB: Hela and K562 whole cell lysate. IHC-P: Human glioma tissue. ICC/IF: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200 - 1/500.
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IHC-P |
1/500 - 1/1000.
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WB |
1/500 - 1/5000. Predicted molecular weight: 89 kDa.
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Notes |
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ICC/IF
1/200 - 1/500. |
IHC-P
1/500 - 1/1000. |
WB
1/500 - 1/5000. Predicted molecular weight: 89 kDa. |
Target
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Function
Microtubule-based anterograde translocator for membranous organelles. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin II subfamily.
Contains 1 kinesin-motor domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 3797 Human
- Entrez Gene: 16570 Mouse
- Entrez Gene: 85248 Rat
- Omim: 602845 Human
- SwissProt: A0JN40 Cow
- SwissProt: O14782 Human
- SwissProt: O35066 Mouse
- SwissProt: Q3UT07 Mouse
see all -
Alternative names
- KIAA4058 antibody
- KIF3C antibody
- KIF3C variant protein antibody
see all
Images
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All lanes : Anti-KIF3C antibody (ab236748) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/500 dilution
Predicted band size: 89 kDa -
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling KIF3C (green) using ab236748 at 1/200 dilution in ICC/IFF, follwed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody. Counterstained with DAPI (blue).
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Paraffin-embedded human glioma tissue stained for KIF3C using ab236748 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236748 has been referenced in 1 publication.
- Liu H et al. Kinesin family member 3C (KIF3C) is a novel non-small cell lung cancer (NSCLC) oncogene whose expression is modulated by microRNA-150-5p (miR-150-5p) and microRNA-186-3p (miR-186-3p). Bioengineered 12:3077-3088 (2021). PubMed: 34193018