Key features and details
- Rabbit polyclonal to KIF3C
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KIF3C antibody
DescriptionRabbit polyclonal to KIF3C
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
- WB: Hela and K562 whole cell lysate. IHC-P: Human glioma tissue. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/500.|
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 89 kDa.|
FunctionMicrotubule-based anterograde translocator for membranous organelles.
Sequence similaritiesBelongs to the kinesin-like protein family. Kinesin II subfamily.
Contains 1 kinesin-motor domain.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- KIAA4058 antibody
- KIF3C antibody
- KIF3C variant protein antibody
All lanes : Anti-KIF3C antibody (ab236748) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/500 dilution
Predicted band size: 89 kDa
4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling KIF3C (green) using ab236748 at 1/200 dilution in ICC/IFF, follwed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody. Counterstained with DAPI (blue).
Paraffin-embedded human glioma tissue stained for KIF3C using ab236748 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236748 has not yet been referenced specifically in any publications.