• Product name
    Anti-KIF4A antibody [EPR5459]
    See all KIF4A primary antibodies
  • Description
    Rabbit monoclonal [EPR5459] to KIF4A
  • Host species
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human KIF4A aa 50-150. The exact sequence is proprietary.

  • Positive control
    • Mouse thymus tissue lysate; HeLa cell lysate; 293T cell lysate; 293 cells
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function
      Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
    • Tissue specificity
      Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
    • Sequence similarities
      Belongs to the kinesin-like protein family. Chromokinesin subfamily.
      Contains 1 kinesin-motor domain.
    • Cellular localization
      Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Chromokinesin antibody
      • Chromokinesin-A antibody
      • Chromosome associated kinesin KIF4A antibody
      • Chromosome-associated kinesin KIF4A antibody
      • FLJ12530 antibody
      • FLJ12655 antibody
      • FLJ14204 antibody
      • FLJ20631 antibody
      • HSA271784 antibody
      • KIF4 antibody
      • KIF4 G1 antibody
      • KIF4A antibody
      • KIF4A_HUMAN antibody
      • KIF4G1 antibody
      • Kinesin family member 4A antibody
      see all


    • All lanes : Anti-KIF4A antibody [EPR5459] (ab124903) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 140 kDa
      Observed band size: 170 kDa (why is the actual band size different from the predicted?)

    • Flow cytometric analysis of permeablized 293 cells using ab124903 at 1/10 dilution (red) or a rabbit IgG negative control (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Willemsen MH  et al. Involvement of the kinesin family members KIF4A and KIF5C in intellectual disability and synaptic function. J Med Genet 51:487-94 (2014). Read more (PubMed: 24812067) »

    See 1 Publication for this product

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