For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    kif4akif4-antibody-ab3815.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Cell Cycle Cell Division Spindle
Share by email

Anti-KIF4A/KIF4 antibody (ab3815)

  • Datasheet
Reviews (5)Q&A (6)References (6)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-KIF4A/KIF4 antibody (ab3815)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (ab3815)

Key features and details

  • Goat polyclonal to KIF4A/KIF4
  • Suitable for: WB, ICC
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-KIF4A/KIF4 antibody
    See all KIF4A/KIF4 primary antibodies
  • Description

    Goat polyclonal to KIF4A/KIF4
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, ICCmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig
  • Immunogen

    Synthetic peptide corresponding to Human KIF4A/KIF4 aa 1219-1232 (C terminal).
    Sequence:

    SFFSGCSPIEEEAH


    (Peptide available as ab22955)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: NIH/3T3 nuclear lysate. ICC: U-2 OS cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Cell Biology
    • Apoptosis
    • Nucleus
    • PARP
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Scaffold Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • PARP

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3815 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.
ICC
Use a concentration of 10 µg/ml.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.
ICC
Use a concentration of 10 µg/ml.

Target

  • Function

    Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.
  • Tissue specificity

    Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
  • Sequence similarities

    Belongs to the kinesin-like protein family. Chromokinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization

    Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
  • Target information above from: UniProt accession O95239 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 24137 Human
    • Entrez Gene: 16571 Mouse
    • Entrez Gene: 84393 Rat
    • NCBI: 7305205 Human
    • Omim: 300521 Human
    • SwissProt: O95239 Human
    • SwissProt: P33174 Mouse
    • Unigene: 648326 Human
    • Alternative names

      • Chromokinesin antibody
      • Chromokinesin-A antibody
      • Chromosome associated kinesin KIF4A antibody
      • Chromosome-associated kinesin KIF4A antibody
      • FLJ12530 antibody
      • FLJ12655 antibody
      • FLJ14204 antibody
      • FLJ20631 antibody
      • HSA271784 antibody
      • KIF4 antibody
      • KIF4 G1 antibody
      • KIF4A antibody
      • KIF4A_HUMAN antibody
      • KIF4G1 antibody
      • Kinesin family member 4A antibody
      see all

    Images

    • Western blot - Anti-KIF4A/KIF4 antibody (ab3815)
      Western blot - Anti-KIF4A/KIF4 antibody (ab3815)
      All lanes : Anti-KIF4A/KIF4 antibody (ab3815) at 2 µg/ml

      Lane 1 : NIH/3T3 nuclear lysate
      Lane 2 : Human ovary lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 140 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (ab3815)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (ab3815)

      Immunocytochemical analysis of paraformaldehyde-fixed U-2 OS cells permeabilized with 0.15% Triton. Cells were incubated with ab3815 at 10 ug/ml for 1 hour followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green). The nuclear stain is DAPI (blue). Negative control is unimmunized goat IgG at 10 ug/ml, followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green).

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (6)

    Publishing research using ab3815? Please let us know so that we can cite the reference in this datasheet.

    ab3815 has been referenced in 6 publications.

    • Champion L  et al. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell 30:427-440 (2019). PubMed: 30586323
    • Lopez-Camacho C  et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). PubMed: 24648201
    • Hu CK  et al. Midbody assembly and its regulation during cytokinesis. Mol Biol Cell 23:1024-34 (2012). ICC/IF . PubMed: 22278743
    • Hu CK  et al. Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis. Mol Biol Cell 23:2702-11 (2012). ICC/IF ; Human . PubMed: 22621898
    • Hu CK  et al. KIF4 regulates midzone length during cytokinesis. Curr Biol 21:815-24 (2011). PubMed: 21565503
    • Wilmeth LJ  et al. Mutual dependence of Mob1 and the chromosomal passenger complex for localization during mitosis. Mol Biol Cell 21:380-92 (2010). Human . PubMed: 19955215

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-5 of 5 Abreviews

    Immunohistochemistry (Frozen sections) abreview for Anti-KIF4A/KIF4 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (retina)
    Permeabilization
    Yes - triton-X100
    Specification
    retina
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 28 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KIF4A/KIF4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cell line)
    Permeabilization
    Yes - triton .15
    Specification
    HeLa cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 09 2012

    Immunoprecipitation abreview for Anti-KIF4A/KIF4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Total protein in input
    10 µg
    Treatment
    500nM nocodazole for 12 hrs
    Immuno-precipitation step
    Protein A/G
    Specification
    HeLa cells
    Read More

    Abcam user community

    Verified customer

    Submitted May 28 2009

    Western blot abreview for Anti-KIF4A/KIF4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Treatment
    Nocodazole synchronized for 12hrs
    Specification
    HeLa
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 23 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KIF4A antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 03 2008

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2022 Abcam plc. All rights reserved.