Anti-KIF4A/KIF4 antibody (ab3815)
Key features and details
- Goat polyclonal to KIF4A/KIF4
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-KIF4A/KIF4 antibody
See all KIF4A/KIF4 primary antibodies -
Description
Goat polyclonal to KIF4A/KIF4 -
Host species
Goat -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human KIF4A/KIF4 aa 1219-1232 (C terminal).
Sequence:SFFSGCSPIEEEAH
(Peptide available asab22955) -
Positive control
- WB: NIH/3T3 nuclear lysate. ICC: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa.
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| ICC |
Use a concentration of 10 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 140 kDa. |
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ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
Tissue specificity
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
Sequence similarities
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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Database links
- Entrez Gene: 24137 Human
- Entrez Gene: 16571 Mouse
- Entrez Gene: 84393 Rat
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- SwissProt: P33174 Mouse
- Unigene: 648326 Human
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Alternative names
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
Images
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Western blot - Anti-KIF4A/KIF4 antibody (ab3815)All lanes : Anti-KIF4A/KIF4 antibody (ab3815) at 2 µg/ml
Lane 1 : NIH/3T3 nuclear lysate
Lane 2 : Human ovary lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 140 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF4A/KIF4 antibody (ab3815)Immunocytochemical analysis of paraformaldehyde-fixed U-2 OS cells permeabilized with 0.15% Triton. Cells were incubated with ab3815 at 10 ug/ml for 1 hour followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green). The nuclear stain is DAPI (blue). Negative control is unimmunized goat IgG at 10 ug/ml, followed by an Alexa Fluor® 488 secondary at 4 ug/ml (green).
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab3815 has been referenced in 6 publications.
- Champion L et al. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell 30:427-440 (2019). PubMed: 30586323
- Lopez-Camacho C et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). PubMed: 24648201
- Hu CK et al. Midbody assembly and its regulation during cytokinesis. Mol Biol Cell 23:1024-34 (2012). ICC/IF . PubMed: 22278743
- Hu CK et al. Plk1 negatively regulates PRC1 to prevent premature midzone formation before cytokinesis. Mol Biol Cell 23:2702-11 (2012). ICC/IF ; Human . PubMed: 22621898
- Hu CK et al. KIF4 regulates midzone length during cytokinesis. Curr Biol 21:815-24 (2011). PubMed: 21565503
- Wilmeth LJ et al. Mutual dependence of Mob1 and the chromosomal passenger complex for localization during mitosis. Mol Biol Cell 21:380-92 (2010). Human . PubMed: 19955215
