Anti-KIF4A/KIF4 antibody (ab3815)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-KIF4A/KIF4 antibody
See all KIF4A/KIF4 primary antibodies -
Description
Goat polyclonal to KIF4A/KIF4 -
Host species
Goat -
Tested applications
Suitable for: IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human KIF4A/KIF4 aa 1219-1232 (C terminal).
Sequence:SFFSGCSPIEEEAH
(Peptide available asab22955)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab3815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | 1/100. Can be blocked with Human KIF4A/KIF4 peptide (ab22955). | |
| ICC/IF | 1/100. |
Target
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Function
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
Tissue specificity
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
Sequence similarities
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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Database links
- Entrez Gene: 24137 Human
- Entrez Gene: 16571 Mouse
- Entrez Gene: 84393 Rat
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- SwissProt: P33174 Mouse
- Unigene: 648326 Human
see all -
Alternative names
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-KIF4A/KIF4 antibody (ab3815)This image is courtesy of an anonymous Abreviewab3815 (1/100) stainning KIF4A/KIF4 in HeLa Cells (blue). Cells were fixed with Methanol and BSA was used as blocking agent. Please refer to abreview for further experimental details.
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Western blot - Anti-KIF4A/KIF4 antibody (ab3815)Image courtesy of an anonymous Abreview.All lanes : Anti-KIF4A/KIF4 antibody (ab3815) at 1/100 dilution
Lane 1 : Whole cell lysate prepared from HeLa cells synchronized with Nocodazole for 12 hours.
Lane 2 : As Lane one but with KIF4A/KIF4 siRNA treatment as negative control.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated anti goat at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Protocols
Datasheets and documents
References
This product has been referenced in:
- Champion L et al. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell 30:427-440 (2019). Read more (PubMed: 30586323) »
- Lopez-Camacho C et al. Core binding factor ß (CBFß) is retained in the midbody during cytokinesis. J Cell Physiol 229:1466-74 (2014). Read more (PubMed: 24648201) »
