Western blot abreview for Anti-KIF4A/KIF4 antibody

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Treatment
Nocodazole synchronized for 12hrs
Specification
HeLa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 27°C · Diluent: PBST
Dilution
1/100

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti goat HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 140 kDa

Additional data

Additional Notes
HeLa cells were synchronized by nocodazole for overnight. Then this mitotic enriched lysate was blotted with anti-KIF4a antibody. A ~140kD band was observed as predicted. Lane 1: Nocodazole enriched mitotic HeLa extract Lane 2: Same as lane 1 but with KIF4A siRNA treatment as negative control

Abcam user community

Verified customer

Submitted Mar 23 2009

Sign up