Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa cells)
Total protein in input
10 µg
Treatment
500nM nocodazole for 12 hrs
Immuno-precipitation step
Protein A/G
Specification
HeLa cells
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Concentration
2.5 µg/mg lysate
Additional data
Additional Notes
lane 1: lysate for IP
lane 2: IP
Blotted with Abcam KIF4 antibody (ab3815), The same membrane later was striped and reblotted with another KIF4 antibody (BETHYL, A301), showing bands are indeed KIF4.
Abcam user community
Verified customer
Submitted May 28 2009