Anti-KIF4A/KIF4 antibody (ab72412)
Key features and details
- Rabbit polyclonal to KIF4A/KIF4
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF4A/KIF4 antibody
See all KIF4A/KIF4 primary antibodies -
Description
Rabbit polyclonal to KIF4A/KIF4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Pig, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human KIF4A/KIF4 (C terminal).
Database link: NP_036442.3 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72412 was affinity purified using an epitope specific to KIF4A/KIF4 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72412 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
Tissue specificity
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
Sequence similarities
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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Database links
- Entrez Gene: 24137 Human
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- Unigene: 648326 Human
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Alternative names
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
Images
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All lanes : Anti-KIF4A/KIF4 antibody (ab72412) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 140 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Detection of KIF4A/KIF4 by Western Blot of Immunprecipitate.
ab72412 at 1µg/ml staining KIF4A/KIF4 in HeLa whole cell lysate immunoprecipitated using ab72412 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded).
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab72412 has not yet been referenced specifically in any publications.