Recombinant Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
![Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)](https://www.abcam.com/ps/products/124/ab124903/Images/ab124903-138879-anti-kif4a-antibody-epr5459-western-blot.jpg "Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5459] to KIF4A/KIF4
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-KIF4A/KIF4 antibody [EPR5459]
See all KIF4A/KIF4 primary antibodies -
Description
Rabbit monoclonal [EPR5459] to KIF4A/KIF4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human KIF4A/KIF4 aa 50-150. The exact sequence is proprietary.
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Positive control
- Mouse thymus tissue lysate; HeLa cell lysate; 293T cell lysate; 293 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.52 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5459 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa). |
Application notes
Is unsuitable for ICC,IHC-P or IP.
Target
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Function
Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization. -
Tissue specificity
Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung. -
Sequence similarities
Belongs to the kinesin-like protein family. Chromokinesin subfamily.
Contains 1 kinesin-motor domain. -
Cellular localization
Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis. - Information by UniProt
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Database links
- Entrez Gene: 24137 Human
- Entrez Gene: 16571 Mouse
- Entrez Gene: 84393 Rat
- NCBI: 7305205 Human
- Omim: 300521 Human
- SwissProt: O95239 Human
- SwissProt: P33174 Mouse
- Unigene: 648326 Human
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Alternative names
- Chromokinesin antibody
- Chromokinesin-A antibody
- Chromosome associated kinesin KIF4A antibody
see all
Images
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Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)All lanes : Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903) at 1/1000 dilution
Lane 1 : Mouse thymus tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 140 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)](https://www.abcam.com/ps/products/124/ab124903/Images/ab124903-138878-anti-kif4a-antibody-epr5459-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)Intracellular flow cytometric analysis of permeablized 293 cells using ab124903 at 1/10 dilution (red) or a rabbit IgG negative control (green).
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![OI-RD Scanning - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)](https://www.abcam.com/ps/products/124/ab124903/Images/ab124903-138877-anti-kif4a-antibody-epr5459.jpg)
OI-RD Scanning - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903) -
![Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)](https://www.abcam.com/ps/products/124/ab124903/Images/ab124903-4-benefits-of-recombinant-antibodies.png)
Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab124903 has been referenced in 10 publications.
- Sun X et al. KIF4A enhanced cell proliferation and migration via Hippo signaling and predicted a poor prognosis in esophageal squamous cell carcinoma. Thorac Cancer 12:512-524 (2021). PubMed: 33350074
- Song Y et al. Identification of KIF4A and its effect on the progression of lung adenocarcinoma based on the bioinformatics analysis. Biosci Rep 41:N/A (2021). PubMed: 33398330
- Zhang DY et al. KIF4A as a novel prognostic biomarker in cholangiocarcinoma. Medicine (Baltimore) 100:e26130 (2021). PubMed: 34032761
- Cui X et al. Increased expression of KPNA2 predicts unfavorable prognosis in ovarian cancer patients, possibly by targeting KIF4A signaling. J Ovarian Res 14:71 (2021). PubMed: 34034774
- Cao Q et al. Targeting the KIF4A/AR Axis to Reverse Endocrine Therapy Resistance in Castration-resistant Prostate Cancer. Clin Cancer Res 26:1516-1528 (2020). PubMed: 31796514
- Yang D et al. Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation. Cell Death Dis 11:508 (2020). PubMed: 32641713
- Hu G et al. FOXM1 promotes hepatocellular carcinoma progression by regulating KIF4A expression. J Exp Clin Cancer Res 38:188 (2019). PubMed: 31072351
- Wei YL & Yang WX Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance. Cell Death Dis 10:402 (2019). PubMed: 31127080
- Su KC et al. A Regulatory Switch Alters Chromosome Motions at the Metaphase-to-Anaphase Transition. Cell Rep 17:1728-1738 (2016). PubMed: 27829144
- Willemsen MH et al. Involvement of the kinesin family members KIF4A and KIF5C in intellectual disability and synaptic function. J Med Genet 51:487-94 (2014). PubMed: 24812067