RecombinantRabMAb

Recombinant Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5459] to KIF4A/KIF4
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-KIF4A/KIF4 antibody [EPR5459]
    See all KIF4A/KIF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR5459] to KIF4A/KIF4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human KIF4A/KIF4 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Mouse thymus tissue lysate; HeLa cell lysate; 293T cell lysate; 293 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab124903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 140 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Motor protein that translocates PRC1 to the plus ends of interdigitating spindle microtubules during the metaphase to anaphase transition, an essential step for the formation of an organized central spindle midzone and midbody and for successful cytokinesis. May play a role in mitotic chromosomal positioning and bipolar spindle stabilization.

    • Tissue specificity

      Highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.

    • Sequence similarities

      Belongs to the kinesin-like protein family. Chromokinesin subfamily.
      Contains 1 kinesin-motor domain.

    • Cellular localization

      Nucleus matrix. Cytoplasm > cytoskeleton > spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Chromokinesin antibody
      • Chromokinesin-A antibody
      • Chromosome associated kinesin KIF4A antibody
      • Chromosome-associated kinesin KIF4A antibody
      • FLJ12530 antibody
      • FLJ12655 antibody
      • FLJ14204 antibody
      • FLJ20631 antibody
      • HSA271784 antibody
      • KIF4 antibody
      • KIF4 G1 antibody
      • KIF4A antibody
      • KIF4A_HUMAN antibody
      • KIF4G1 antibody
      • Kinesin family member 4A antibody
      see all

    Images

    • Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
      Western blot - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
      All lanes : Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903) at 1/1000 dilution

      Lane 1 : Mouse thymus tissue lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 140 kDa
      Observed band size: 170 kDa
      why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
      Flow Cytometry - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
      Flow cytometric analysis of permeablized 293 cells using ab124903 at 1/10 dilution (red) or a rabbit IgG negative control (green).
    • OI-RD Scanning - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
      OI-RD Scanning - Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)
      Anti-KIF4A/KIF4 antibody [EPR5459] (ab124903)

    References (6)

    Publishing research using ab124903? Please let us know so that we can cite the reference in this datasheet.

    ab124903 has been referenced in 6 publications.

    • Cao Q  et al. Targeting the KIF4A/AR Axis to Reverse Endocrine Therapy Resistance in Castration-resistant Prostate Cancer. Clin Cancer Res 26:1516-1528 (2020). PubMed: 31796514
    • Yang D  et al. Oxidized ATM promotes breast cancer stem cell enrichment through energy metabolism reprogram-mediated acetyl-CoA accumulation. Cell Death Dis 11:508 (2020). PubMed: 32641713
    • Hu G  et al. FOXM1 promotes hepatocellular carcinoma progression by regulating KIF4A expression. J Exp Clin Cancer Res 38:188 (2019). PubMed: 31072351
    • Wei YL & Yang WX Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance. Cell Death Dis 10:402 (2019). PubMed: 31127080
    • Su KC  et al. A Regulatory Switch Alters Chromosome Motions at the Metaphase-to-Anaphase Transition. Cell Rep 17:1728-1738 (2016). PubMed: 27829144
    • Willemsen MH  et al. Involvement of the kinesin family members KIF4A and KIF5C in intellectual disability and synaptic function. J Med Genet 51:487-94 (2014). PubMed: 24812067

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