Key features and details
- Rabbit polyclonal to KIF5A + KIF5B + KIF5C
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KIF5A + KIF5B + KIF5C antibody
See all KIF5A + KIF5B + KIF5C primary antibodies
DescriptionRabbit polyclonal to KIF5A + KIF5B + KIF5C
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human KIF5A + KIF5B + KIF5C (C terminal).
Database link: Q12840
This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab62104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 116 kDa (predicted molecular weight: 110 kDa).|
RelevanceThe kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.
Cellular localizationUniformly distributed between soma and neurites in hippocampal neurons
- KIF5A antibody
- KIF5B antibody
- KIF5C antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse brain tissue staining KIF5A + KIF5B + KIF5C with ab62104 at 20µg/ml
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling KIF5A + KIF5B + KIF5C with Anti-KIF5A + KIF5B + KIF5C antibody (ab62104) at 5μg/ml.
Lane 1 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62104) at 0.5 µg/ml
Lane 2 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62104) at 1 µg/ml
All lanes : K562 cell lysate
Predicted band size: 110 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted?
ab62104 at 5 µg/ml staining KIF5A + KIF5B + KIF5C in human K562 cells by Immunocytochemistry.
Immunofluorescence of KIF5 in K562 cells using ab62104 at 20 ug/ml.
ab62104 has been referenced in 4 publications.
- Thillaiappan NB et al. Ca2+signals initiate at immobile IP3receptors adjacent to ER-plasma membrane junctions. Nat Commun 8:1505 (2017). PubMed: 29138405
- Farías GG et al. BORC/kinesin-1 ensemble drives polarized transport of lysosomes into the axon. Proc Natl Acad Sci U S A 114:E2955-E2964 (2017). PubMed: 28320970
- Sherman MA et al. Soluble Conformers of Aß and Tau Alter Selective Proteins Governing Axonal Transport. J Neurosci 36:9647-58 (2016). PubMed: 27629715
- Su B et al. Brain-derived neurotrophic factor (BDNF)-induced mitochondrial motility arrest and presynaptic docking contribute to BDNF-enhanced synaptic transmission. J Biol Chem 289:1213-26 (2014). Rat . PubMed: 24302729