Anti-KIF5A + KIF5B + KIF5C antibody (ab62105)
Key features and details
- Rabbit polyclonal to KIF5A + KIF5B + KIF5C
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF5A + KIF5B + KIF5C antibody
See all KIF5A + KIF5B + KIF5C primary antibodies -
Description
Rabbit polyclonal to KIF5A + KIF5B + KIF5C -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human KIF5A + KIF5B + KIF5C.
Database link: Q12840 -
General notes
This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab62105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 110 kDa). |
Target
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Relevance
The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias. -
Cellular localization
Uniformly distributed between soma and neurites in hippocampal neurons -
Database links
- Entrez Gene: 3798 Human
- Entrez Gene: 3799 Human
- Entrez Gene: 3800 Human
- SwissProt: O60282 Human
- SwissProt: P33176 Human
- SwissProt: Q12840 Human
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Alternative names
- KIF5A antibody
- KIF5B antibody
- KIF5C antibody
see all
Images
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Western blot - Anti-KIF5A + KIF5B + KIF5C antibody (ab62105)Lane 1 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62105) at 0.5 µg/ml
Lane 2 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62105) at 1 µg/ml
All lanes : K562 cell lysate
Predicted band size: 110 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-KIF5A + KIF5B + KIF5C antibody (ab62105)ab62105 at 5µg/ml staining KIF5A + KIF5B + KIF5C in human K562 cells by Immunohistochemistry.
Datasheets and documents
References (1)
ab62105 has been referenced in 1 publication.
- Zollo M et al. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. Brain 140:940-952 (2017). WB . PubMed: 28334956
