Anti-KIF5A + KIF5B + KIF5C antibody (ab62105)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-KIF5A + KIF5B + KIF5C antibody
See all KIF5A + KIF5B + KIF5C primary antibodies -
Description
Rabbit polyclonal to KIF5A + KIF5B + KIF5C -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human KIF5A + KIF5B + KIF5C.
Database link: Q12840 -
General notes
This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab62105 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| ICC | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 110 kDa). |
Target
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Relevance
The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias. -
Cellular localization
Uniformly distributed between soma and neurites in hippocampal neurons -
Database links
- Entrez Gene: 3798 Human
- Entrez Gene: 3799 Human
- Entrez Gene: 3800 Human
- SwissProt: O60282 Human
- SwissProt: P33176 Human
- SwissProt: Q12840 Human
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Alternative names
- KIF5A antibody
- KIF5B antibody
- KIF5C antibody
see all
Images
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Western blot - Anti-KIF5A + KIF5B + KIF5C antibody (ab62105)Lane 1 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62105) at 0.5 µg/ml
Lane 2 : Anti-KIF5A + KIF5B + KIF5C antibody (ab62105) at 1 µg/ml
All lanes : K562 cell lysate
Predicted band size: 110 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-KIF5A + KIF5B + KIF5C antibody (ab62105)ab62105 at 5µg/ml staining KIF5A + KIF5B + KIF5C in human K562 cells by Immunohistochemistry.
Datasheets and documents
References
This product has been referenced in:
- Zollo M et al. PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. Brain 140:940-952 (2017). WB . Read more (PubMed: 28334956) »
