Recombinant Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (ab271049)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24325-98] to KIF5A + KIF5B + KIF5C
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98]
See all KIF5A + KIF5B + KIF5C primary antibodies -
Description
Rabbit monoclonal [EPR24325-98] to KIF5A + KIF5B + KIF5C -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Neuro-2a, C2C12, U-87 MG, SH-SY5Y, Rat brain cortex, C6, Mouse brain, Mouse cerebral cortex, Mouse cerebellum, Rat brain, Rat cerebellum, Human cerebellum, Human hypothalamus, HEK-293T transfected with KIF5A, KIF5B and KIF5C expression vector containing a myc-His-tag® whole cell lysates. ICC/IF: mouse primary neuron and rat primary neuron, SH-SY5Y cells. Flow Cyt: SH-SY5Y, Neuro-2a and C6 cells. IP: Neuro-2a, SH-SY5Y cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24325-98 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/500.
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WB |
1/1000. Predicted molecular weight: 117 kDa.
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IP |
1/30.
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ICC/IF |
1/50.
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Notes |
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Flow Cyt (Intra)
1/500. |
WB
1/1000. Predicted molecular weight: 117 kDa. |
IP
1/30. |
ICC/IF
1/50. |
Target
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Relevance
The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias. -
Cellular localization
Uniformly distributed between soma and neurites in hippocampal neurons -
Database links
- Entrez Gene: 3798 Human
- Entrez Gene: 3799 Human
- Entrez Gene: 3800 Human
- SwissProt: O60282 Human
- SwissProt: P33176 Human
- SwissProt: Q12840 Human
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Alternative names
- KIF5A antibody
- KIF5B antibody
- KIF5C antibody
see all
Images
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All lanes : Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (ab271049) at 1/1000 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate
Lane 2 : C2C12 (mouse myoblasts myoblast), whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate
Lane 5 : Rat brain cortex tissue lysate
Lane 6 : C6 (rat glial tumor glial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 10 seconds
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All lanes : Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (ab271049) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebral cortex tissue lysate
Lane 3 : Mouse cerebellum tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat cerebellum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 117 kDa
Observed band size: 117,107 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Degraded fragment (107 KD) has also been described (PMID: 11144355)
Exposure time: 10 seconds
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All lanes : Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (ab271049) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human hypothalamus tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 117 kDa
Observed band size: 117,107 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Degraded fragment (107 KD) has also been described (PMID: 11144355)
Exposure time: 10 seconds
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All lanes : Anti-KIF5A + KIF5B + KIF5C antibody [EPR24325-98] (ab271049) at 1/5000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with KIF5A expression vector containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with KIF5B expression vector containing a myc-His-tag®, whole cell lysate
Lane 3 : HEK-293T transfected with KIF5C expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 2 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 117 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 seconds
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling KIF5A + KIF5B + KIF5C with ab271049 at 1/50 (9.72 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4 ug/ml) dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling KIF5A + KIF5B + KIF5C with ab271049 at 1/50 (9.72 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4 ug/ml) dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling KIF5A + KIF5B + KIF5C with ab271049 at 1/50 (9.72 ug/ml) dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 (4 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line. is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 (4 ug/ml) dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling KIF5A + KIF5B + KIF5C with ab271049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling KIF5A + KIF5B + KIF5C with ab271049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C6 (Rat glial tumor glial cell) cells labelling KIF5A + KIF5B + KIF5C with ab271049 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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KIF5A + KIF5B + KIF5C was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with ab271049 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271049 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug
Lane 2: ab271049 IP in Neuro-2a whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271049 in Neuro-2a whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
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KIF5A + KIF5B + KIF5C was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug with ab271049 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271049 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 10 ug
Lane 2: ab271049 IP in SH-SY5Y whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271049 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab271049 has not yet been referenced specifically in any publications.