Anti-KIF5A antibody (ab154378)
Key features and details
- Rabbit polyclonal to KIF5A
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF5A antibody
See all KIF5A primary antibodies -
Description
Rabbit polyclonal to KIF5A -
Host species
Rabbit -
Tested Applications & Species
Application Species WB MouseHuman -
Immunogen
Recombinant fragment corresponding to Human KIF5A aa 508-800.
Database link: Q12840 -
Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysates.
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General notes
This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
Mouse
Human
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All applications |
Rat
Cow
Xenopus tropicalis
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Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 117 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa. |
Target
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Function
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). -
Tissue specificity
Distributed throughout the CNS but is highly enriched in subsets of neurons. -
Involvement in disease
Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 511273 Cow
- Entrez Gene: 3798 Human
- Entrez Gene: 16572 Mouse
- Entrez Gene: 314906 Rat
- Entrez Gene: 100124766 Xenopus tropicalis
- Omim: 602821 Human
- SwissProt: Q12840 Human
- SwissProt: P33175 Mouse
see all -
Alternative names
- D12S1889 antibody
- KIF 5A antibody
- Kif5a antibody
see all
Images
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Anti-KIF5A antibody (ab154378) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 117 kDa
7.5% SDS PAGE -
Anti-KIF5A antibody (ab154378) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 117 kDa
7.5% SDS PAGE
References (0)
ab154378 has not yet been referenced specifically in any publications.