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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Kinesin
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Anti-KIF5A antibody (ab154378)

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  • SDS
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Western blot - Anti-KIF5A antibody (ab154378)
  • Western blot - Anti-KIF5A antibody (ab154378)

Key features and details

  • Rabbit polyclonal to KIF5A
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-KIF5A antibody
    See all KIF5A primary antibodies
  • Description

    Rabbit polyclonal to KIF5A
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human KIF5A aa 508-800.
    Database link: Q12840

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji and Mouse brain whole cell lysates.
  • General notes

     This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • Mouse brain tissue lysate - total protein (ab30151)
    • A-431 whole cell lysate (ab7909)
    • MOLT-4 whole cell lysate (ab7912)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Human
All applications
Rat
Cow
Xenopus tropicalis
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa.

Target

  • Function

    Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • Tissue specificity

    Distributed throughout the CNS but is highly enriched in subsets of neurons.
  • Involvement in disease

    Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • Sequence similarities

    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization

    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Target information above from: UniProt accession Q12840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 511273 Cow
    • Entrez Gene: 3798 Human
    • Entrez Gene: 16572 Mouse
    • Entrez Gene: 314906 Rat
    • Entrez Gene: 100124766 Xenopus tropicalis
    • Omim: 602821 Human
    • SwissProt: Q12840 Human
    • SwissProt: P33175 Mouse
    • SwissProt: Q6QLM7 Rat
    • Unigene: 151219 Human
    • Unigene: 30355 Mouse
    • Unigene: 63144 Rat
    see all
  • Alternative names

    • D12S1889 antibody
    • KIF 5A antibody
    • Kif5a antibody
    • KIF5A_HUMAN antibody
    • Kinesin family member 5A antibody
    • Kinesin heavy chain isoform 5A antibody
    • Kinesin Heavy Chain Neuron Specific antibody
    • Kinesin heavy chain neuron-specific 1 antibody
    • MY050 antibody
    • Neuronal kinesin heavy chain antibody
    • NKHC 1 antibody
    • NKHC antibody
    • SPG 10 antibody
    see all

Images

  • Western blot - Anti-KIF5A antibody (ab154378)
    Western blot - Anti-KIF5A antibody (ab154378)
    Anti-KIF5A antibody (ab154378) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 117 kDa



    7.5% SDS PAGE
  • Western blot - Anti-KIF5A antibody (ab154378)
    Western blot - Anti-KIF5A antibody (ab154378)
    Anti-KIF5A antibody (ab154378) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 117 kDa



    7.5% SDS PAGE

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154378? Please let us know so that we can cite the reference in this datasheet.

    ab154378 has not yet been referenced specifically in any publications.

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