Overview

  • Product name

  • Description

    Rabbit polyclonal to KIF5A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human KIF5A aa 969-1032 (C terminal).
    Database link: Q12840

  • Positive control

    • Human IMR32 and Mouse brain lysates; HeLa cells; Human Cal27 xenograft tissue.
  • General notes

     This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 117 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • Tissue specificity

    Distributed throughout the CNS but is highly enriched in subsets of neurons.
  • Involvement in disease

    Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • Sequence similarities

    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization

    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • D12S1889 antibody
    • KIF 5A antibody
    • Kif5a antibody
    • KIF5A_HUMAN antibody
    • Kinesin family member 5A antibody
    • Kinesin heavy chain isoform 5A antibody
    • Kinesin Heavy Chain Neuron Specific antibody
    • Kinesin heavy chain neuron-specific 1 antibody
    • MY050 antibody
    • Neuronal kinesin heavy chain antibody
    • NKHC 1 antibody
    • NKHC antibody
    • SPG 10 antibody
    see all

Images

  • Anti-KIF5A antibody (ab154414) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 117 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KIF5A using ab154414 at a 1/500 dilution.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Cal27 xenograft tissue, labeling KIF5A using ab154414 at a 1/100 dilution.

  • Anti-KIF5A antibody (ab154414) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg

    Predicted band size: 117 kDa



    7.5% SDS PAGE

References

ab154414 has not yet been referenced specifically in any publications.

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