• Product name
  • Description
    Rabbit polyclonal to KIF5A
  • Host species
  • Specificity

    This antibody is specific for KIF5A and does not detect other kinesin isotypes.

  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse KIF5A aa 1007-1027.


    (Peptide available as ab41781)

  • Positive control
    • Mouse neuronal cell extract.
  • General notes

     This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C



  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab5628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration. PubMed: 20094756
WB Use a concentration of 0.4 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 117 kDa).Can be blocked with KIF5A peptide (ab41781).


  • Function
    Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • Tissue specificity
    Distributed throughout the CNS but is highly enriched in subsets of neurons.
  • Involvement in disease
    Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain
    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization
    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • D12S1889 antibody
    • KIF 5A antibody
    • Kif5a antibody
    • KIF5A_HUMAN antibody
    • Kinesin family member 5A antibody
    • Kinesin heavy chain isoform 5A antibody
    • Kinesin Heavy Chain Neuron Specific antibody
    • Kinesin heavy chain neuron-specific 1 antibody
    • MY050 antibody
    • Neuronal kinesin heavy chain antibody
    • NKHC 1 antibody
    • NKHC antibody
    • SPG 10 antibody
    see all


  • Anti-KIF5A antibody (ab5628) + mouse neuronal cell extract

    HRP-conjugated anti rabbit antibody

    Predicted band size: 117 kDa

    Shows a Western blot of KIF5A on mouse neuronal cell extract using ab5628.

  • Anti-KIF5A antibody (ab5628) + human retinal extract

    HRP-conjugated anti-rabbit antibody

    Predicted band size: 117 kDa

  • ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Kamata H  et al. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis. Sci Rep 7:4556 (2017). WB . Read more (PubMed: 28676645) »
  • Blakeslee WW  et al. Class I HDACs control a JIP1-dependent pathway for kinesin-microtubule binding in cardiomyocytes. J Mol Cell Cardiol 112:74-82 (2017). WB . Read more (PubMed: 28886967) »
See all 7 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


Dear Abcam technical team, I want to order proteins from your website, but there are some questions before order. (1) I want to experiment with conventional kinesin heavy chain, KIF5. But there are three different members of kinesin family 5. KIF5A, KIF5B and KIF5C. I know the function of each member, A and C involves in neuronal transport otherwise B involves in inner cell transport. But is there any structural difference between three members? If is, I have to choose specific member to experiment. (2) There are two types in KIF5A and three types in KIF5B. It seems that each has different domain, so I wonder there are functional or structural difference between types. Also, your company has two different types of KIF5C. One is just (938-957) domain and the other is His-tagged protein. His tagged kinesin has higher protein purity. Is there any reason to choose His tagged KIF5C except higher purity? (3) Is antibody that has specificity of my protein (e.g. KIF5A --> Anti-kinesin 5A antibody) attach to specific domain (like C-terminal) or it is just randomly attached? For my experiment, it is good for antibody to attach to C-terminal. In other words, is antibody has C-terminal specificity? (4) Do you sell biotinylated antibody for kinesin? I want antibody that has biotin coating but your website has no option or products for biotin coating. If you cannot understand my questions, please let me know which parts you cannot understand. Then I will send the more specific explanation for my questions. From

Read More

Thank you for contacting us and your interest in our products. 1.) Indeed, there is structural differences between those members. The sequences of the three members are not the same. I have made an alignment where you can see these differences. Please refer to the following link to see these differences: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111011-085702-0894-35538860-oy To check out further parameters of these proteins, I can recommend to browse either the expasy site (http://web.expasy.org/ or http://web.expasy.org/protparam/) as well as one of the Bioinformatics institutes homepages, for example the EBI, http://www.ebi.ac.uk/. There are different Tools to predict the protein structures. 2.) We do have currently only one KIF5 protein in our catalogue, and this is the ab91695 Kinesin 5C protein (Tagged-His Tag) which is not a full length protein but a recombinant fragment corresponding to amino acids 500-957 of Human Kinesin 5C. Click here (or use the following: https://www.abcam.com/index.html?datasheet=91695). All the other products are indeed peptides and do correspond to the short amino acid sequence of the numbers in brackets. These peptides are too short to be able to have a function similar to the whole protein, however they can be used for blocking studies with the corresponding antibody. For example ab41784 is a peptide corresponding the sequence 938 to 957 of human KIF5C. Similarly for the other products: KIF5A: - peptide amino acid 1019-1032 is ab23133 and - peptide amino acid 1007-1027 is ab41781 KIF5B - peptide amino acid 376-396 is ab41782 - peptide amino acid 951-963 is ab23262 - peptide ab100817, a few amino acids derived from the sequence between amino acid 350 to 450 3.) Each antibody is different. I have therefore put together the following list: KIF5A, we do have two antibodies: -ab5628 is a polyclonal antibody directed against the C terminus of KIF5A, recognizing the sequences of amino acids 1007 to 1027 of KIF5A. This antibody has also been tested against the KIF5A protein of different species (human, mouse, rat and cow) as well as in immunocytochemistry, immunoprecipitation and Western blot. Click here (or use the following: https://www.abcam.com/index.html?datasheet=5628). -ab5958 which is a polyclonal antibody directed against the C terminal sequence (amino acids 1019 - 1032 of KIF5A). This antibody has however not been well characterized as yet, as you see it is one of our Fasttrack antibodies. Please let me know if you would like to know more about this. Click here (or use the following: https://www.abcam.com/index.html?datasheet=5958). KIF5B we have 7 antibodies currently: two of them are against a region in the C terminal: ab15705, corresponding to amino acids 951-963 Click here (or use the following: https://www.abcam.com/index.html?datasheet=15705). ab97762, corresponding to amino acids 901 - 963 Click here (or use the following: https://www.abcam.com/index.html?datasheet=97762). The other KIF5B antibodies are either N terminal (ab42492, immunogen amino acids 7-56), from the central region (ab5629, immunogen amino acids 376-396 and ab91033 region derived from amino acids 350 - 450) or are mouse monoclonal antibodies for which we do not know the epitope (ab110155 and ab11262) KIF5C we only have one antibody: ab5630 which has been raised against a C terminal sequence as well, the amino acids 938 - 957 of the human KIF5C. Click here (or use the following: https://www.abcam.com/index.html?datasheet=5630). 4.) Indeed we do currently not have one of these antibodies in biotinylated form. We do have however the Easylink conjugation kits, which are especially designed for easy conjugation of antibodies. We do have such kits also to perform biotin conjugation. You can find more information in this regards under the following website: www.abcam.com/easylink Again, please let me know if you wish more information about this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Western blot
Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
Loading amount
10 µg
Human SH-SY5Y neuroblastoma cells
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Mrs. Adelheid Hanes

Verified customer

Submitted Nov 15 2010

Rat Tissue lysate - whole (Forebrain)
Total protein in input
20000 µg
Immuno-precipitation step
Protein A

Dr. Kieran Brickley

Verified customer

Submitted May 06 2009

Western blot
Rat Cell lysate - whole cell (Rat whole brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Rat whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 15 2009

For licensing inquiries, please contact partnerships@abcam.com

Sign up