Anti-KIF5A antibody (ab5628)
Key features and details
- Rabbit polyclonal to KIF5A
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF5A antibody
See all KIF5A primary antibodies -
Description
Rabbit polyclonal to KIF5A -
Host species
Rabbit -
Specificity
This antibody is specific for KIF5A and does not detect other kinesin isotypes.
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Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse KIF5A aa 1007-1027.
Sequence:CGYEAEDQAKLFPLHQETAAS
(Peptide available asab41781) -
General notes
This product was previously labelled as Kinesin 5A, Kinesin 5 A + B + C
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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| WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 117 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 1 - 5 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 117 kDa. |
Target
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Function
Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). -
Tissue specificity
Distributed throughout the CNS but is highly enriched in subsets of neurons. -
Involvement in disease
Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
Cellular localization
Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 3798 Human
- Entrez Gene: 16572 Mouse
- Entrez Gene: 314906 Rat
- Omim: 602821 Human
- SwissProt: Q12840 Human
- SwissProt: P33175 Mouse
- SwissProt: Q6QLM7 Rat
- Unigene: 151219 Human
see all -
Alternative names
- D12S1889 antibody
- KIF 5A antibody
- Kif5a antibody
see all
Images
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Immunocytochemistry - Anti-KIF5A antibody (ab5628)
Immunofluorescence analysis of ADAMTS9 in Kinesin 5A cells using ab5628. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab5628 at 1 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing cytoplasmic localization. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.
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Western blot - Anti-KIF5A antibody (ab5628)All lanes : Anti-KIF5A antibody (ab5628) at 2 µg/ml
Lane 1 : U-87 MG whole cell extracts
Lane 2 : HT-29 whole cell extracts
Lane 3 : C2C12 whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution
Predicted band size: 117 kDaBlocking with 5 % skimmed milk
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Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (11)
ab5628 has been referenced in 11 publications.
- Furey C et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
- Zhao J et al. Specific depletion of the motor protein KIF5B leads to deficits in dendritic transport, synaptic plasticity and memory. Elife 9:N/A (2020). PubMed: 31961321
- Shi L et al. Coupling of microtubule motors with AP-3 generated organelles in axons by NEEP21 family member calcyon. Mol Biol Cell 29:2055-2068 (2018). PubMed: 29949458
- Kamata H et al. KIF5A transports collagen vesicles of myofibroblasts during pleural fibrosis. Sci Rep 7:4556 (2017). WB . PubMed: 28676645
- Blakeslee WW et al. Class I HDACs control a JIP1-dependent pathway for kinesin-microtubule binding in cardiomyocytes. J Mol Cell Cardiol 112:74-82 (2017). WB . PubMed: 28886967
