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    kif5a-antibody-ab5628.pdf

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Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Microtubules
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Anti-KIF5A antibody (ab5628)

  • Datasheet
Reviews (3)Q&A (1)References (14)

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Immunocytochemistry - Anti-KIF5A antibody (ab5628)
  • Western blot - Anti-KIF5A antibody (ab5628)
  • Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)

Key features and details

  • Rabbit polyclonal to KIF5A
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-KIF5A antibody
    See all KIF5A primary antibodies
  • Description

    Rabbit polyclonal to KIF5A
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for KIF5A and does not detect other kinesin isotypes.

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse KIF5A aa 1007-1027.
    Sequence:

    CGYEAEDQAKLFPLHQETAAS


    (Peptide available as ab41781)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5628 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB (2)
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 117 kDa.
IP (1)
Use at an assay dependent concentration. PubMed: 20094756
Notes
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 117 kDa.
IP
Use at an assay dependent concentration. PubMed: 20094756

Target

  • Function

    Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL).
  • Tissue specificity

    Distributed throughout the CNS but is highly enriched in subsets of neurons.
  • Involvement in disease

    Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10) [MIM:604187]. An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
  • Sequence similarities

    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization

    Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Concentrated in the cell body of the neurons, particularly in the perinuclear region.
  • Target information above from: UniProt accession Q12840 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 511273 Cow
    • Entrez Gene: 3798 Human
    • Entrez Gene: 16572 Mouse
    • Entrez Gene: 314906 Rat
    • Omim: 602821 Human
    • SwissProt: Q12840 Human
    • SwissProt: P33175 Mouse
    • SwissProt: Q6QLM7 Rat
    • Unigene: 151219 Human
    • Unigene: 30355 Mouse
    • Unigene: 63144 Rat
    see all
  • Alternative names

    • D12S1889 antibody
    • KIF 5A antibody
    • Kif5a antibody
    • KIF5A_HUMAN antibody
    • Kinesin family member 5A antibody
    • Kinesin heavy chain isoform 5A antibody
    • Kinesin Heavy Chain Neuron Specific antibody
    • Kinesin heavy chain neuron-specific 1 antibody
    • MY050 antibody
    • Neuronal kinesin heavy chain antibody
    • NKHC 1 antibody
    • NKHC antibody
    • SPG 10 antibody
    see all

Images

  • Immunocytochemistry - Anti-KIF5A antibody (ab5628)
    Immunocytochemistry - Anti-KIF5A antibody (ab5628)

    Immunofluorescence analysis of ADAMTS9 in Kinesin 5A cells using ab5628. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab5628 at 1 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature followed by a Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin at 1/300. (Panel d) represents the merged image showing cytoplasmic localization. (Panel e) shows the no primary antibody control. The images were captured at 60X magnification.

  • Western blot - Anti-KIF5A antibody (ab5628)
    Western blot - Anti-KIF5A antibody (ab5628)
    All lanes : Anti-KIF5A antibody (ab5628) at 2 µg/ml

    Lane 1 : U-87 MG whole cell extracts
    Lane 2 : HT-29 whole cell extracts
    Lane 3 : C2C12 whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 1/2500 dilution

    Predicted band size: 117 kDa



    Blocking with 5 % skimmed milk

  • Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)
    Immunocytochemistry/ Immunofluorescence - Anti-KIF5A antibody (ab5628)
    ICC/IF image of ab5628 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5628, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (14)

Publishing research using ab5628? Please let us know so that we can cite the reference in this datasheet.

ab5628 has been referenced in 14 publications.

  • Liu M  et al. KIF5A-dependent axonal transport deficiency disrupts autophagic flux in trimethyltin chloride-induced neurotoxicity. Autophagy 17:903-924 (2021). PubMed: 32160081
  • Xie MX  et al. ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking. Nat Commun 12:1401 (2021). PubMed: 33658516
  • Brachet A  et al. A kinesin 1-protrudin complex mediates AMPA receptor synaptic removal during long-term depression. Cell Rep 36:109499 (2021). PubMed: 34348158
  • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
  • Zhao J  et al. Specific depletion of the motor protein KIF5B leads to deficits in dendritic transport, synaptic plasticity and memory. Elife 9:N/A (2020). PubMed: 31961321
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Kinesin 5A antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
Loading amount
10 µg
Specification
Human SH-SY5Y neuroblastoma cells
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Mrs. Adelheid Hanes

Verified customer

Submitted Nov 15 2010

Western blot abreview for Anti-KIF5A antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat whole brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Rat whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More

Abcam user community

Verified customer

Submitted Apr 15 2009

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