Western blot abreview for Anti-KIF5A antibody

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat whole brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Rat whole brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 115 kDa

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Submitted Apr 15 2009

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