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Rat Tissue lysate - whole (Forebrain)
Total protein in input
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: HBS, containing 0.5% Triton X-100
Lysys buffer was 10mM HEPES, pH, 7.4, containing 145mM NaCl, 1mM EGTA, 0.1mM MgCl2, 1% (v/v) Triton X-100 [(10 micrograms/ml) benzamidine, soybean trypsin inhibitor, chicken egg trypsin inhibitor, bacitracin and phenylmethyl sulfonic acid. Proteins were transferred to nitrocellulose at 14V for 16h at rt.
Dr. Kieran Brickley
Submitted May 06 2009