Immunoprecipitation abreview for Anti-Kinesin 5A antibody

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (Forebrain)
Total protein in input
20000 µg
Specification
Forebrain
Immuno-precipitation step
Protein A

Other product details

Dilution
1/4000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: HBS, containing 0.5% Triton X-100

Western Blot antibody

Name
Dilution
1/2000

Additional data

Additional Notes
Lysys buffer was 10mM HEPES, pH, 7.4, containing 145mM NaCl, 1mM EGTA, 0.1mM MgCl2, 1% (v/v) Triton X-100 [(10 micrograms/ml) benzamidine, soybean trypsin inhibitor, chicken egg trypsin inhibitor, bacitracin and phenylmethyl sulfonic acid. Proteins were transferred to nitrocellulose at 14V for 16h at rt.

Dr. Kieran Brickley

Verified customer

Submitted May 06 2009

Sign up