Western blot abreview for Anti-Kinesin 5A antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
Loading amount
10 µg
Specification
Human SH-SY5Y neuroblastoma cells
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3 % marvel/TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit HRP conjugated secondary
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 110 kDa

Mrs. Adelheid Hanes

Verified customer

Submitted Nov 15 2010

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