Key features and details
- Rabbit polyclonal to KIF5B
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-KIF5B antibody
See all KIF5B primary antibodies
DescriptionRabbit polyclonal to KIF5B
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
- HeLa, 293T, Jurkat and TCMK1 whole cell lysates.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab187693 was affinity purified using an epitope specific to Kinesin Heavy Chain immobilized on solid support.
Our Abpromise guarantee covers the use of ab187693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/500 - 1/2500. Predicted molecular weight: 110 kDa.|
FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
Contains 1 kinesin motor domain.
DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
- Information by UniProt
- Conventional kinesin heavy chain antibody
- KIF 5B antibody
- KIF5B antibody
All lanes : Anti-KIF5B antibody (ab187693) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK1 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 110 kDa
Exposure time: 30 seconds
Detection of Kinesin Heavy Chain in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab187693 at 6 µg/mg lysate. An alternative anti-Kinesin Heavy Chain antibody was used at 1 µg/ml for subsequent western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 second.
ab187693 has not yet been referenced specifically in any publications.