Overview

  • Product name
  • Description
    Rabbit polyclonal to KIF5B
  • Host species
    Rabbit
  • Specificity
    This antibody detects total level of endogenous kinesin protein.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Recombinant fragment corresponding to Human KIF5B. Stalk domain of human KIF5B (amino acids 331-906) expressed in E. coli (FKHC3).

Properties

Applications

Our Abpromise guarantee covers the use of ab25715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
WB 1/1000. Predicted molecular weight: 110 kDa.

Target

  • Function
    Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
  • Sequence similarities
    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
    Contains 1 kinesin motor domain.
  • Domain
    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization
    Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • Conventional kinesin heavy chain antibody
    • KIF 5B antibody
    • KIF5B antibody
    • Kinesin 1 antibody
    • kinesin 1 (110-120kD) antibody
    • Kinesin 1 heavy chain antibody
    • Kinesin family member 5B antibody
    • Kinesin heavy chain antibody
    • kinesin, heavy chain, ubiquitous antibody
    • Kinesin-1 heavy chain antibody
    • Kinesin1 antibody
    • KINH antibody
    • KINH_HUMAN antibody
    • KNS 1 antibody
    • KNS antibody
    • KNS1 antibody
    • Ubiquitous kinesin heavy chain antibody
    • UKHC antibody
    see all

Images

  • All lanes : Anti-KIF5B antibody (ab25715)

    Lane 1 : Human HEK cell line
    Lane 2 : Porcine brain

    Predicted band size: 110 kDa

  • ab25715 staining KIF5B in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilzed with 0.1% Triton and blocked with 0.1% BSA for 16 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS + 0.1% BSA) for 1 hour at 37°C. An Alexa Fluor®546-conjugated donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). Read more (PubMed: 28930676) »
  • Malikov V  et al. HIV-1 capsids bind and exploit the kinesin-1 adaptor FEZ1 for inward movement to the nucleus. Nat Commun 6:6660 (2015). WB, ICC/IF . Read more (PubMed: 25818806) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cells)
Specification
HeLa Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Apr 22 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (A549 Cells)
Loading amount
250 µg
Specification
A549 Cells
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 01 2008

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