Western blot abreview for Anti-Kinesin antibody

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 Cells)
Loading amount
250 µg
Specification
A549 Cells
Gel Running Conditions
Non-reduced Denaturing (4-20% Tris-Glycine Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in pbs with 0.5% tween

Secondary antibody

Name
Non-Abcam antibody was used: IRDYE 680 donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 700DX
Dilution
1/5000

Detection

Detection method
Licor Odyssey
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 125 kDa Non-specific: 100, 80, 30 kDa
Negative control
Lane 1: BSA, and Lane 2: Sample Buffer

Additional data

Additional Notes
Lane 1: BSA Lane 2: Sample Buffer Lane 3: Cell Lysate. There are a few background bands, but the most prominent band is the kinesin at around 110-125kD.
Ladder top to bottom is: 250kD, 150kD, 100 kD, 75 kD (very light), 50kD, 37kD, 25kD, etc.

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Submitted Oct 01 2008

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