Anti-KIF5B antibody [EPR10277(B)] (ab151558)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-KIF5B antibody [EPR10277(B)]
See all KIF5B primary antibodies -
DescriptionRabbit monoclonal [EPR10277(B)] to KIF5B
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human KIF5B aa 700-800. The exact sequence is proprietary.
Database link: P33176 -
Positive control
- Jurkat, HeLa and HepG2 cell lysates; Human colon and uterus tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20ºC.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10277(B)
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | 1/1000 - 1/10000. Predicted molecular weight: 111 kDa. | |
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | 1/100 - 1/250. |
Target
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FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
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Sequence similaritiesBelongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
Contains 1 kinesin motor domain. -
DomainComposed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
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Cellular localizationCytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
- Information by UniProt
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Database links
- Entrez Gene: 3799 Human
- Omim: 602809 Human
- SwissProt: P33176 Human
- Unigene: 327736 Human
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Alternative names
- Conventional kinesin heavy chain antibody
- KIF 5B antibody
- KIF5B antibody
see all
Images
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![Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)](https://www.abcam.com/ps/products/151/ab151558/Images/ab151558-291916-ab151558GDBKOWB.jpg)
Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: KIF5B knockout HAP1 whole cell lysate (20 µg)
Lane 3: Hek293 whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab151558 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab151558 was shown to specifically react with KIF5B in wild type cells as signal was lost in KIF5B knockout cells. Wild-type and KIF5B knockout samples were subjected to SDS-PAGE. Ab151558 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry - Anti-KIF5B antibody [EPR10277(B)] (ab151558)](https://www.abcam.com/ps/products/151/ab151558/Images/ab151558-4-ab151558FC.jpg)
Flow Cytometry - Anti-KIF5B antibody [EPR10277(B)] (ab151558)Overlay histogram showing HeLa cells stained with ab151558 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151558, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. -
![Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)](https://www.abcam.com/ps/products/151/ab151558/Images/KIF5B-Primary-antibodies-ab151558-1.jpg)
Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)All lanes : Anti-KIF5B antibody [EPR10277(B)] (ab151558) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 111 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)](https://www.abcam.com/ps/products/151/ab151558/Images/KIF5B-Primary-antibodies-ab151558-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling KIF5B with ab151558 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)](https://www.abcam.com/ps/products/151/ab151558/Images/KIF5B-Primary-antibodies-ab151558-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling KIF5B with ab151558 at 1/50 dilution.
Protocols
References
This product has been referenced in:
- Raiborg C et al. Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth. Nature 520:234-8 (2015). Read more (PubMed: 25855459) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"