Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-KIF5B antibody [EPR10277(B)] (ab151558)

Reviews (1)Q&A (1)References (1)

Overview

  • Product name

    Anti-KIF5B antibody [EPR10277(B)]
    See all KIF5B primary antibodies

  • Description

    Rabbit monoclonal [EPR10277(B)] to KIF5B

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human KIF5B aa 700-800. The exact sequence is proprietary.
    Database link: P33176

  • Positive control

    • Jurkat, HeLa and HepG2 cell lysates; Human colon and uterus tissues.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10277(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 111 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.

  • Sequence similarities

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.
    Contains 1 kinesin motor domain.

  • Domain

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.

  • Cellular localization

    Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal neurons.
  • Information by UniProt

  • Database links

    • Alternative names

      • Conventional kinesin heavy chain antibody
      • KIF 5B antibody
      • KIF5B antibody
      • Kinesin 1 antibody
      • kinesin 1 (110-120kD) antibody
      • Kinesin 1 heavy chain antibody
      • Kinesin family member 5B antibody
      • Kinesin heavy chain antibody
      • kinesin, heavy chain, ubiquitous antibody
      • Kinesin-1 heavy chain antibody
      • Kinesin1 antibody
      • KINH antibody
      • KINH_HUMAN antibody
      • KNS 1 antibody
      • KNS antibody
      • KNS1 antibody
      • Ubiquitous kinesin heavy chain antibody
      • UKHC antibody
      see all

    Images

    • Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)
      Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: KIF5B knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Hek293 whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab151558 observed at 110 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab151558 was shown to specifically react with KIF5B in wild type cells as signal was lost in KIF5B knockout cells. Wild-type and KIF5B knockout samples were subjected to SDS-PAGE. Ab151558 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-KIF5B antibody [EPR10277(B)] (ab151558)
      Flow Cytometry - Anti-KIF5B antibody [EPR10277(B)] (ab151558)
      Overlay histogram showing HeLa cells stained with ab151558 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab151558, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)
      Western blot - Anti-KIF5B antibody [EPR10277(B)] (ab151558)
      All lanes : Anti-KIF5B antibody [EPR10277(B)] (ab151558) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 111 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling KIF5B with ab151558 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5B antibody [EPR10277(B)] (ab151558)

      Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling KIF5B with ab151558 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Publishing research using ab151558? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KIF5B antibody [EPR10277(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (mesenchymal cells)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    mesenchymal cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 23 2018

    Question

    Has this antibody been tested with mouse samples?

    Read More
    Answer

    ab151558 was tested against the following mouse samples, with no reactivity detected:

    brain tissue lysate
    heart tissue lysate
    kidney tissue lysate
    spleen tissue lysate
    Raw 264.7 lysate
    NIH/3T3 cell lysate

    Read More

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