Anti-KIF5C antibody (ab5630)
Key features and details
- Rabbit polyclonal to KIF5C
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF5C antibody
See all KIF5C primary antibodies -
Description
Rabbit polyclonal to KIF5C -
Host species
Rabbit -
Specificity
This antibody is specific for KIF5C and does not detect other kinesin isotypes.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human KIF5C aa 938-957.
Sequence:AVHAIRGGGGSSSNSTHYQK
(Peptide available asab41784) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
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| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (4) |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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| Notes |
|---|
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
Target
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Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. -
Tissue specificity
Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 3800 Human
- Omim: 604593 Human
- SwissProt: O60282 Human
- Unigene: 435557 Human
- Unigene: 660699 Human
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Alternative names
- CDCBM2 antibody
- FLJ44735 antibody
- KIAA0531 antibody
see all
Images
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Western blot - Anti-KIF5C antibody (ab5630)
Shows a Western blot of KIF5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab5630 has been referenced in 10 publications.
- Furey C et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
- Zhao J et al. Specific depletion of the motor protein KIF5B leads to deficits in dendritic transport, synaptic plasticity and memory. Elife 9:N/A (2020). PubMed: 31961321
- Pan X et al. MAP7D2 Localizes to the Proximal Axon and Locally Promotes Kinesin-1-Mediated Cargo Transport into the Axon. Cell Rep 26:1988-1999.e6 (2019). PubMed: 30784582
- Leiva-Rodríguez T et al. ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons. Cell Death Dis 9:626 (2018). PubMed: 29799519
- Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). PubMed: 28930676
- Du W et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell 37:326-336 (2016). PubMed: 27219061
- Poirier K et al. Mutations in TUBG1, DYNC1H1, KIF5C and KIF2A cause malformations of cortical development and microcephaly. Nat Genet 45:639-47 (2013). PubMed: 23603762
- Dunn S et al. Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells. J Cell Sci 121:1085-95 (2008). ICC/IF ; Monkey . PubMed: 18334549
- Smith MJ et al. Mapping the GRIF-1 binding domain of the kinesin, KIF5C, substantiates a role for GRIF-1 as an adaptor protein in the anterograde trafficking of cargoes. J Biol Chem 281:27216-28 (2006). PubMed: 16835241
- Brickley K et al. GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin. J Biol Chem 280:14723-32 (2005). PubMed: 15644324