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    kif5c-antibody-ab5630.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Motor Proteins Kinesin
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Anti-KIF5C antibody (ab5630)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (10)

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Western blot - Anti-KIF5C antibody (ab5630)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)

Key features and details

  • Rabbit polyclonal to KIF5C
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-KIF5C antibody
    See all KIF5C primary antibodies
  • Description

    Rabbit polyclonal to KIF5C
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for KIF5C and does not detect other kinesin isotypes.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human KIF5C aa 938-957.
    Sequence:

    AVHAIRGGGGSSSNSTHYQK


    (Peptide available as ab41784)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Kinesin

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (4)
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Notes
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

Target

  • Function

    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • Tissue specificity

    Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
  • Sequence similarities

    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession O60282 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3800 Human
    • Omim: 604593 Human
    • SwissProt: O60282 Human
    • Unigene: 435557 Human
    • Unigene: 660699 Human
    • Alternative names

      • CDCBM2 antibody
      • FLJ44735 antibody
      • KIAA0531 antibody
      • KIF 5C antibody
      • Kif5c antibody
      • KIF5C protein antibody
      • KIF5C_HUMAN antibody
      • Kinesin family member 5C antibody
      • Kinesin heavy chain isoform 5C antibody
      • Kinesin heavy chain neuron specific 2 antibody
      • Kinesin heavy chain neuron specific antibody
      • Kinesin heavy chain neuron-specific 2 antibody
      • Kinesin5C antibody
      • KINN antibody
      • MGC111478 antibody
      • NKHC 2 antibody
      • NKHC antibody
      • NKHC-2 antibody
      • NKHC2 antibody
      see all

    Images

    • Western blot - Anti-KIF5C antibody (ab5630)
      Western blot - Anti-KIF5C antibody (ab5630)

      Shows a Western blot of KIF5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF5C antibody (ab5630)
      IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (10)

    Publishing research using ab5630? Please let us know so that we can cite the reference in this datasheet.

    ab5630 has been referenced in 10 publications.

    • Furey C  et al. TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells. Cell Rep 30:269-283.e6 (2020). PubMed: 31914393
    • Zhao J  et al. Specific depletion of the motor protein KIF5B leads to deficits in dendritic transport, synaptic plasticity and memory. Elife 9:N/A (2020). PubMed: 31961321
    • Pan X  et al. MAP7D2 Localizes to the Proximal Axon and Locally Promotes Kinesin-1-Mediated Cargo Transport into the Axon. Cell Rep 26:1988-1999.e6 (2019). PubMed: 30784582
    • Leiva-Rodríguez T  et al. ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons. Cell Death Dis 9:626 (2018). PubMed: 29799519
    • Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). PubMed: 28930676
    • Du W  et al. Kinesin 1 Drives Autolysosome Tubulation. Dev Cell 37:326-336 (2016). PubMed: 27219061
    • Poirier K  et al. Mutations in TUBG1, DYNC1H1, KIF5C and KIF2A cause malformations of cortical development and microcephaly. Nat Genet 45:639-47 (2013). PubMed: 23603762
    • Dunn S  et al. Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells. J Cell Sci 121:1085-95 (2008). ICC/IF ; Monkey . PubMed: 18334549
    • Smith MJ  et al. Mapping the GRIF-1 binding domain of the kinesin, KIF5C, substantiates a role for GRIF-1 as an adaptor protein in the anterograde trafficking of cargoes. J Biol Chem 281:27216-28 (2006). PubMed: 16835241
    • Brickley K  et al. GRIF-1 and OIP106, members of a novel gene family of coiled-coil domain proteins: association in vivo and in vitro with kinesin. J Biol Chem 280:14723-32 (2005). PubMed: 15644324

    Customer reviews and Q&As

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    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-KIF5C antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris precast gel)
    Loading amount
    5 µg
    Specification
    Brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 24 2019

    Question

    Dear Abcam technical team, I want to order proteins from your website, but there are some questions before order. (1) I want to experiment with conventional kinesin heavy chain, KIF5. But there are three different members of kinesin family 5. KIF5A, KIF5B and KIF5C. I know the function of each member, A and C involves in neuronal transport otherwise B involves in inner cell transport. But is there any structural difference between three members? If is, I have to choose specific member to experiment. (2) There are two types in KIF5A and three types in KIF5B. It seems that each has different domain, so I wonder there are functional or structural difference between types. Also, your company has two different types of KIF5C. One is just (938-957) domain and the other is His-tagged protein. His tagged kinesin has higher protein purity. Is there any reason to choose His tagged KIF5C except higher purity? (3) Is antibody that has specificity of my protein (e.g. KIF5A --> Anti-kinesin 5A antibody) attach to specific domain (like C-terminal) or it is just randomly attached? For my experiment, it is good for antibody to attach to C-terminal. In other words, is antibody has C-terminal specificity? (4) Do you sell biotinylated antibody for kinesin? I want antibody that has biotin coating but your website has no option or products for biotin coating. If you cannot understand my questions, please let me know which parts you cannot understand. Then I will send the more specific explanation for my questions. From

    Read More

    Abcam community

    Verified customer

    Asked on Oct 11 2011

    Answer

    Thank you for contacting us and your interest in our products. 1.) Indeed, there is structural differences between those members. The sequences of the three members are not the same. I have made an alignment where you can see these differences. Please refer to the following link to see these differences: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111011-085702-0894-35538860-oy To check out further parameters of these proteins, I can recommend to browse either the expasy site (http://web.expasy.org/ or http://web.expasy.org/protparam/) as well as one of the Bioinformatics institutes homepages, for example the EBI, http://www.ebi.ac.uk/. There are different Tools to predict the protein structures. 2.) We do have currently only one KIF5 protein in our catalogue, and this is the ab91695 Kinesin 5C protein (Tagged-His Tag) which is not a full length protein but a recombinant fragment corresponding to amino acids 500-957 of Human Kinesin 5C. Click here (or use the following: https://www.abcam.com/Human-Kinesin-5C-protein-fragment-ab91695.html). All the other products are indeed peptides and do correspond to the short amino acid sequence of the numbers in brackets. These peptides are too short to be able to have a function similar to the whole protein, however they can be used for blocking studies with the corresponding antibody. For example ab41784 is a peptide corresponding the sequence 938 to 957 of human KIF5C. Similarly for the other products: KIF5A: - peptide amino acid 1019-1032 is ab23133 and - peptide amino acid 1007-1027 is ab41781 KIF5B - peptide amino acid 376-396 is ab41782 - peptide amino acid 951-963 is ab23262 - peptide ab100817, a few amino acids derived from the sequence between amino acid 350 to 450 3.) Each antibody is different. I have therefore put together the following list: KIF5A, we do have two antibodies: -ab5628 is a polyclonal antibody directed against the C terminus of KIF5A, recognizing the sequences of amino acids 1007 to 1027 of KIF5A. This antibody has also been tested against the KIF5A protein of different species (human, mouse, rat and cow) as well as in immunocytochemistry, immunoprecipitation and Western blot. Click here (or use the following: https://www.abcam.com/KIF5A-antibody-ab5628.html). -ab5958 which is a polyclonal antibody directed against the C terminal sequence (amino acids 1019 - 1032 of KIF5A). This antibody has however not been well characterized as yet, as you see it is one of our Fasttrack antibodies. Please let me know if you would like to know more about this. Click here (or use the following: https://www.abcam.com/KIF5A-antibody-ab5958.html). KIF5B we have 7 antibodies currently: two of them are against a region in the C terminal: ab15705, corresponding to amino acids 951-963 Click here (or use the following: https://www.abcam.com/Kinesin-5B-antibody-ab15705.html). ab97762, corresponding to amino acids 901 - 963 Click here (or use the following: https://www.abcam.com/KIF5B-antibody-ab97762.html). The other KIF5B antibodies are either N terminal (ab42492, immunogen amino acids 7-56), from the central region (ab5629, immunogen amino acids 376-396 and ab91033 region derived from amino acids 350 - 450) or are mouse monoclonal antibodies for which we do not know the epitope (ab110155 and ab11262) KIF5C we only have one antibody: ab5630 which has been raised against a C terminal sequence as well, the amino acids 938 - 957 of the human KIF5C. Click here (or use the following: https://www.abcam.com/KIF5C-antibody-ab5630.html). 4.) Indeed we do currently not have one of these antibodies in biotinylated form. We do have however the Easylink conjugation kits, which are especially designed for easy conjugation of antibodies. We do have such kits also to perform biotin conjugation. You can find more information in this regards under the following website: www.abcam.com/easylink Again, please let me know if you wish more information about this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 11 2011

    Western blot abreview for Anti-KIF5C antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    Human SH-SY5Y neuroblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Mrs. Adelheid Hanes

    Verified customer

    Submitted Nov 16 2010

    Immunoprecipitation abreview for Anti-KIF5C antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Rat Tissue lysate - whole (forebrain)
    Immuno-precipitation step
    Protein A
    Specification
    forebrain
    Read More

    Dr. Kieran Brickley

    Verified customer

    Submitted May 14 2009

    Western blot abreview for Anti-Kinesin 5C antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Recombinant protein (HEK 293 cells)
    Loading amount
    50 µg
    Specification
    HEK 293 cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2009

    Western blot abreview for Anti-KIF5C antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Brain)
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Loading amount
    100 µg
    Specification
    Brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 27 2009

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