Overview

  • Product name

    Anti-KIF5C antibody [EPR16224-32]
    See all KIF5C primary antibodies
  • Description

    Rabbit monoclonal [EPR16224-32] to KIF5C
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human KIF5C aa 600-850. The exact sequence is proprietary.
    Database link: O60282

  • Positive control

    • Neuro-2a, Human fetal brain, SH-SY5Y, mouse brain, rat brain and C6 lysate. Human testis tissue. Mouse cerebral cortex tissue. SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16224-32
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab193352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/150.
WB 1/1000 - 1/10000. Predicted molecular weight: 109 kDa.
IHC-P 1/150 - 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • Tissue specificity

    Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
  • Sequence similarities

    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain

    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization

    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links

  • Alternative names

    • CDCBM2 antibody
    • FLJ44735 antibody
    • KIAA0531 antibody
    • KIF 5C antibody
    • Kif5c antibody
    • KIF5C protein antibody
    • KIF5C_HUMAN antibody
    • Kinesin family member 5C antibody
    • Kinesin heavy chain isoform 5C antibody
    • Kinesin heavy chain neuron specific 2 antibody
    • Kinesin heavy chain neuron specific antibody
    • Kinesin heavy chain neuron-specific 2 antibody
    • Kinesin5C antibody
    • KINN antibody
    • MGC111478 antibody
    • NKHC 2 antibody
    • NKHC antibody
    • NKHC-2 antibody
    • NKHC2 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde fixed SH-SY5Y cells labeling KIF5C using ab193352 at a 1/150 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/400 dilution. Counterstain DAPI. Cells were permeabilized using 0.1% Triton X-100.

  • All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/10000 dilution

    Lane 1 : Neuro-2a lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : SH-SY5Y lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

  • All lanes : Anti-KIF5C antibody [EPR16224-32] (ab193352) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 109 kDa

  • Immunohistochemical analysis of paraffin embedded Human testis tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue sections labeling KIF5C using ab193352 at a 1/300 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

ab193352 has not yet been referenced specifically in any publications.

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