Recombinant Anti-KIF5C antibody [EPR16224] (ab192883)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16224] to KIF5C
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-KIF5C antibody [EPR16224]
See all KIF5C primary antibodies -
Description
Rabbit monoclonal [EPR16224] to KIF5C -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Neuro-2a, Human fetal brain, SH-SY5Y, K562, rat brain, C6 and PC12 cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16224 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa). |
Target
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Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. -
Tissue specificity
Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 3800 Human
- Entrez Gene: 16574 Mouse
- Entrez Gene: 311024 Rat
- Omim: 604593 Human
- SwissProt: O60282 Human
- SwissProt: P28738 Mouse
- SwissProt: P56536 Rat
- Unigene: 435557 Human
see all -
Alternative names
- CDCBM2 antibody
- FLJ44735 antibody
- KIAA0531 antibody
see all
Images
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All lanes : Anti-KIF5C antibody [EPR16224] (ab192883) at 1/10000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 109 kDa -
Anti-KIF5C antibody [EPR16224] (ab192883) at 1/1000 dilution + K562 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 109 kDa -
All lanes : Anti-KIF5C antibody [EPR16224] (ab192883) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : C6 cell lysate
Lane 3 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 109 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab192883 has not yet been referenced specifically in any publications.