Recombinant Anti-KIF5C antibody [EPR16224] (ab192883)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16224] to KIF5C
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-KIF5C antibody [EPR16224]
See all KIF5C primary antibodies -
Description
Rabbit monoclonal [EPR16224] to KIF5C -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human KIF5C aa 600-850. The exact sequence is proprietary.
Database link: O60282 -
Positive control
- Neuro-2a, Human fetal brain, SH-SY5Y, K562, rat brain, C6 and PC12 cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16224 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab192883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa). |
Target
-
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. -
Tissue specificity
Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin subfamily.
Contains 1 kinesin-motor domain. -
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
-
Database links
- Entrez Gene: 3800 Human
- Entrez Gene: 16574 Mouse
- Entrez Gene: 311024 Rat
- Omim: 604593 Human
- SwissProt: O60282 Human
- SwissProt: P28738 Mouse
- SwissProt: P56536 Rat
- Unigene: 435557 Human
see all -
Alternative names
- CDCBM2 antibody
- FLJ44735 antibody
- KIAA0531 antibody
see all
Images
-
All lanes : Anti-KIF5C antibody [EPR16224] (ab192883) at 1/10000 dilution
Lane 1 : Neuro-2a cell lysate
Lane 2 : Human fetal brain lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 109 kDa -
Anti-KIF5C antibody [EPR16224] (ab192883) at 1/1000 dilution + K562 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 109 kDa -
All lanes : Anti-KIF5C antibody [EPR16224] (ab192883) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : C6 cell lysate
Lane 3 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 109 kDa
Observed band size: 109 kDa
Datasheets and documents
Certificate of Compliance
References (0)
ab192883 has not yet been referenced specifically in any publications.