Overview

  • Product name

  • Description

    Rabbit polyclonal to KIF7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide designed within residues: KRSLCSEGRQ APGNEDELHL APELLWLSPL TEGAPRTREE TRDLVHAPLP L, corresponding to amino acids 1225-1275 of Human KIF7 (NP_940927.2)

  • Positive control

    • HeLa or 293T cell lysate
  • General notes

    Shelf Life: 1 year from date of receipt

Properties

Applications

Our Abpromise guarantee covers the use of ab95884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 151 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Acts as both a negative and positive regulator of sonic hedgehog (Shh) pathway, acting downstream of SMO. Negatively regulates the pathway by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates the pathway by preventing the processing of the transcription factor GLI3 into its repressor form. Required for efficient localization of GLI3 to cilia in response to Shh. May also act as a ciliary motor.
    • Tissue specificity

      Embryonic stem cells, melanotic melanoma and Jurkat T-cells.
    • Sequence similarities

      Belongs to the kinesin-like protein family. KIF27 subfamily.
      Contains 1 kinesin-motor domain.
    • Cellular localization

      Cell projection > cilium. SMO is required for its accumulation within cilia. Moves from the cilia base to the cilia tip in response to activation of the Shh pathway.
    • Information by UniProt
    • Database links

    • Alternative names

      • EQYK340 antibody
      • kif7 antibody
      • KIF7_HUMAN antibody
      • kinesin family member 7 antibody
      • kinesin like protein KIF7 antibody
      • Kinesin-like protein kif7 antibody
      • UNQ340 antibody
      see all

    Images

    • All lanes : Anti-KIF7 antibody (ab95884) at 0.4 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg

      Developed using the ECL technique.

      Predicted band size: 151 kDa


      Exposure time: 30 seconds

    References

    This product has been referenced in:

    • Massa F  et al. The deubiquitinating enzyme USP14 controls ciliogenesis and hedgehog signalling. Hum Mol Genet N/A:N/A (2018). Read more (PubMed: 30388222) »
    • Ho J  et al. Downregulation of the gli transcription factors regulator Kif7 facilitates cell survival and migration of choriocarcinoma cells. PLoS One 9:e108248 (2014). WB ; Human . Read more (PubMed: 25265279) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (10%)
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Mar 03 2014

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