Overview

  • Product name

    Anti-KIF7 antibody - C-terminal
    See all KIF7 primary antibodies
  • Description

    Rabbit polyclonal to KIF7 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human KIF7 (C terminal). The exact sequence is proprietary.
    Database link: Q2M1P5

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab229500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 151 kDa.

Target

  • Function

    Acts as both a negative and positive regulator of sonic hedgehog (Shh) pathway, acting downstream of SMO. Negatively regulates the pathway by preventing inappropriate activation of the transcriptional activator GLI2 in the absence of ligand. Positively regulates the pathway by preventing the processing of the transcription factor GLI3 into its repressor form. Required for efficient localization of GLI3 to cilia in response to Shh. May also act as a ciliary motor.
  • Tissue specificity

    Embryonic stem cells, melanotic melanoma and Jurkat T-cells.
  • Sequence similarities

    Belongs to the kinesin-like protein family. KIF27 subfamily.
    Contains 1 kinesin-motor domain.
  • Cellular localization

    Cell projection > cilium. SMO is required for its accumulation within cilia. Moves from the cilia base to the cilia tip in response to activation of the Shh pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • EQYK340 antibody
    • kif7 antibody
    • KIF7_HUMAN antibody
    • kinesin family member 7 antibody
    • kinesin like protein KIF7 antibody
    • Kinesin-like protein kif7 antibody
    • UNQ340 antibody
    see all

Images

  • All lanes : Anti-KIF7 antibody - C-terminal (ab229500) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 151 kDa



    5% SDS-PAGE

References

ab229500 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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