Product nameAnti-KIFAP3 antibody [EPR5599]
See all KIFAP3 primary antibodies
DescriptionRabbit monoclonal [EPR5599] to KIFAP3
Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human KIFAP3 (N terminal). The exact sequence is proprietary.
- HeLa, SH-SY5Y, C6, 293T, and Human fetal kidney lysates, Human bladder carcinoma tissue
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||1/50 - 1/100.|
FunctionInvolved in tethering the chromosomes to the spindle pole and in chromosome movement. Binds to the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex and may regulate the membrane binding of this complex.
Sequence similaritiesContains 5 ARM repeats.
modificationsPhosphorylated on tyrosine residues by SRC in vitro; this reduces the binding affinity of the protein for RAP1GDS1.
- Information by UniProt
- FLJ22818 antibody
- KAP 3 antibody
- KAP-3 antibody
All lanes : Anti-KIFAP3 antibody [EPR5599] (ab109026) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : C6 lysate
Lane 4 : 293T lysate
Lane 5 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 91 kDa
Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue using ab109026 at a dilution of 1/250.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab109026 has not yet been referenced specifically in any publications.