Overview

  • Product name
    Anti-KIFAP3 antibody [EPR5600]
    See all KIFAP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR5600] to KIFAP3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human KIFAP3 aa 700-800. The exact sequence is proprietary.

  • Positive control
    • HeLa, SH-SY5Y, C6, and 293T cell lysates; and Human kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 100 kDa (predicted molecular weight: 91 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    Images

    • All lanes : Anti-KIFAP3 antibody [EPR5600] (ab133537) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : SH-SY5Y cell lysate
      Lane 3 : C6 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 91 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry analysis of Paraffin Embedded Human kidney tissue labelling KIFAP3 with ab133537 at 1/50
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133537 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133537.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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