Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-KIFC1 antibody [11445] (ab172620)

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Overview

  • Product name

    Anti-KIFC1 antibody [11445]
    See all KIFC1 primary antibodies

  • Description

    Rabbit monoclonal [11445] to KIFC1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human KIFC1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BW19

  • Positive control

    • WB: HeLa, 293T, HepG2 and HAP1 cell lysates IHC-P: Human tonsil tissue, cervical carcinoma, stomachICC/IF: HeLa cells Flow Cyt: HeLa cells IP: Jurkat whole cell lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab172620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
Flow Cyt 1/20.
IHC-P 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols.

Target

  • Function

    Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.

  • Sequence similarities

    Belongs to the kinesin-like protein family. NCD subfamily.
    Contains 1 kinesin-motor domain.

  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis.
  • Information by UniProt

  • Database links

    • Alternative names

      • HSET antibody
      • HSET KNSL2 antibody
      • KIF C1 antibody
      • KIFC 1 antibody
      • KIFC1 antibody
      • KIFC1_HUMAN antibody
      • Kinesin family member C1 antibody
      • Kinesin like 2 antibody
      • Kinesin like protein 2 antibody
      • Kinesin like protein KIFC1 antibody
      • Kinesin related protein HSET antibody
      • Kinesin-like protein 2 antibody
      • Kinesin-like protein KIFC1 antibody
      • Kinesin-related protein HSET antibody
      • KNSL 2 antibody
      • KNSL2 antibody
      • MGC1202 antibody
      • MGC149736 antibody
      • MGC149737 antibody
      • Mitotic kinesin like protein 2 antibody
      • MKLP 2 antibody
      • MKLP2 antibody
      • RAB6 KIFL antibody
      • Rabkinesin 6 antibody
      see all

    Images

    • Western blot - Anti-KIFC1 antibody [11445] (ab172620)
      Western blot - Anti-KIFC1 antibody [11445] (ab172620)
      Anti-KIFC1 antibody [11445] (ab172620) at 1/50000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 74 kDa
      Observed band size: 74 kDa

    • Western blot - Anti-KIFC1 antibody [11445] (ab172620)
      Western blot - Anti-KIFC1 antibody [11445] (ab172620)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: KIFC1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HepG2 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab172620 (purified) observed at 74 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab172620 was shown to specifically react with KIFC1 in wild-type cells as signal was lost in KIFC1 knockout cells. Wild-type and KIFC1 knockout samples were subjected to SDS-PAGE. Ab172620 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.228 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling KIFC1 with purified ab172620 at 1/16000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)
      Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/50 dilution (4.0 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunoprecipitation - Anti-KIFC1 antibody [11445] (ab172620)
      Immunoprecipitation - Anti-KIFC1 antibody [11445] (ab172620)

      ab172620 (purified) at 1/20 dilution (1ug) immunoprecipitating KIFC1 in Jurkat whole cell lysates.
      Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates 10ug
      Lane 2 (+): ab172620 & Jurkat whole cell lysates
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172620 in Jurkat whole cell lysates
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry - Anti-KIFC1 antibody [11445] (ab172620)
      Flow Cytometry - Anti-KIFC1 antibody [11445] (ab172620)

      Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Western blot - Anti-KIFC1 antibody [11445] (ab172620)
      Western blot - Anti-KIFC1 antibody [11445] (ab172620)
      All lanes : Anti-KIFC1 antibody [11445] (ab172620) at 1/10000 dilution ((unpurified))

      Lane 1 : HeLa lysate
      Lane 2 : 293T lysate
      Lane 3 : HepG2 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 74 kDa

    • Western blot - Anti-KIFC1 antibody [11445] (ab172620)
      Western blot - Anti-KIFC1 antibody [11445] (ab172620)

      Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling KIFC1 with ab172620 (unpurified) at 1/10 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue laneling KIFC1 with ab172620 (unpurified) at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)
      Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)

      Immunofluorescent staining of HeLa cells labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.

    References

    Publishing research using ab172620? Please let us know so that we can cite the reference in this datasheet.

    ab172620 has been referenced in 7 publications.

    • Jungwirth G  et al. Identification of KIF11 As a Novel Target in Meningioma. Cancers (Basel) 11:N/A (2019). PubMed: 30991738
    • Teng K  et al. KIFC1 is activated by TCF-4 and promotes hepatocellular carcinoma pathogenesis by regulating HMGA1 transcriptional activity. J Exp Clin Cancer Res 38:329 (2019). PubMed: 31340839
    • Wei YL & Yang WX Kinesin-14 motor protein KIFC1 participates in DNA synthesis and chromatin maintenance. Cell Death Dis 10:402 (2019). PubMed: 31127080
    • She ZY  et al. Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus. Oncotarget 8:36469-36483 (2017). PubMed: 28430595
    • Xiao YX  et al. C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma. Oncotarget 8:61373-61384 (2017). WB ; Human . PubMed: 28977870
    • Marthandan S  et al. Conserved Senescence Associated Genes and Pathways in Primary Human Fibroblasts Detected by RNA-Seq. PLoS One 11:e0154531 (2016). WB . PubMed: 27140416
    • Maurizio E  et al. Translating Proteomic Into Functional Data: An High Mobility Group A1 (HMGA1) Proteomic Signature Has Prognostic Value in Breast Cancer. Mol Cell Proteomics 15:109-23 (2016). IHC . PubMed: 26527623

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