Recombinant Anti-KIFC1 antibody [11445] KO Tested (ab172620)

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Western blot - Anti-KIFC1 antibody [11445] (ab172620)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [11445] to KIFC1
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P
Knockout validated
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free HRP PE

Product name

Anti-KIFC1 antibody [11445]
See all KIFC1 primary antibodies
Description

Rabbit monoclonal [11445] to KIFC1
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human KIFC1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BW19
Positive control
- WB: HeLa, 293T, HepG2 and HAP1 cell lysates IHC-P: Human tonsil tissue, cervical carcinoma, stomachICC/IF: HeLa cells Flow Cyt (intra): HeLa cells IP: Jurkat whole cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

11445
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
- Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
Recombinant Protein
- Recombinant Human KIFC1 protein (ab152492)
Related Products
- Recombinant Human KIFC1 protein (ab152492)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/20.
WB		1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
ICC/IF		1/50 - 1/100.
IP		1/10 - 1/100.
IHC-P		1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols.

Notes
Flow Cyt (Intra) 1/20.
WB 1/10000 - 1/50000. Predicted molecular weight: 74 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
IHC-P 1/16000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/50 - 1/100. See IHC antigen retrieval protocols.

Function

Minus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.
Sequence similarities

Belongs to the kinesin-like protein family. NCD subfamily.
Contains 1 kinesin-motor domain.
Cellular localization

Nucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis.
Target information above from: UniProt accession Q9BW19 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3833 Human
- Omim: 603763 Human
- SwissProt: Q9BW19 Human
- Unigene: 436912 Human
Alternative names
- HSET antibody
- HSET KNSL2 antibody
- KIF C1 antibody
- KIFC 1 antibody
- KIFC1 antibody
- KIFC1_HUMAN antibody
- Kinesin family member C1 antibody
- Kinesin like 2 antibody
- Kinesin like protein 2 antibody
- Kinesin like protein KIFC1 antibody
- Kinesin related protein HSET antibody
- Kinesin-like protein 2 antibody
- Kinesin-like protein KIFC1 antibody
- Kinesin-related protein HSET antibody
- KNSL 2 antibody
- KNSL2 antibody
- MGC1202 antibody
- MGC149736 antibody
- MGC149737 antibody
- Mitotic kinesin like protein 2 antibody
- MKLP 2 antibody
- MKLP2 antibody
- RAB6 KIFL antibody
- Rabkinesin 6 antibody
see all

Western blot - Anti-KIFC1 antibody [11445] (ab172620)

Anti-KIFC1 antibody [11445] (ab172620) at 1/50000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 74 kDa
Observed band size: 74 kDa
Western blot - Anti-KIFC1 antibody [11445] (ab172620)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KIFC1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab172620 (purified) observed at 74 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab172620 was shown to specifically react with KIFC1 in wild-type cells as signal was lost in KIFC1 knockout cells. Wild-type and KIFC1 knockout samples were subjected to SDS-PAGE. Ab172620 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.228 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach tissue sections labeling KIFC1 with purified ab172620 at 1/16000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/50 dilution (4.0 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunoprecipitation - Anti-KIFC1 antibody [11445] (ab172620)

ab172620 (purified) at 1/20 dilution (1ug) immunoprecipitating KIFC1 in Jurkat whole cell lysates.
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates 10ug
Lane 2 (+): ab172620 & Jurkat whole cell lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172620 in Jurkat whole cell lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Flow Cytometry (Intracellular) - Anti-KIFC1 antibody [11445] (ab172620)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling KIFC1 with purified ab172620 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-KIFC1 antibody [11445] (ab172620)

All lanes : Anti-KIFC1 antibody [11445] (ab172620) at 1/10000 dilution ((unpurified))

Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 74 kDa
Western blot - Anti-KIFC1 antibody [11445] (ab172620)

Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling KIFC1 with ab172620 (unpurified) at 1/10 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue laneling KIFC1 with ab172620 (unpurified) at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)

Immunofluorescent staining of HeLa cells labeling KIFC1 with ab172620 (unpurified) at 1/50 dilution.
Anti-KIFC1 antibody [11445] (ab172620)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab172620? Please let us know so that we can cite the reference in this datasheet.

ab172620 has been referenced in 14 publications.

Fan G et al. The ATM and ATR kinases regulate centrosome clustering and tumor recurrence by targeting KIFC1 phosphorylation. Nat Commun 12:20 (2021). PubMed: 33397932
Kostecka LG et al. High KIFC1 expression is associated with poor prognosis in prostate cancer. Med Oncol 38:47 (2021). PubMed: 33760984
Parvin A et al. Inhibition of kinesin motor protein KIFC1 by AZ82 induces multipolar mitosis and apoptosis in prostate cancer cell. Gene 760:144989 (2020). PubMed: 32717307
Begemann D et al. Androgens modify therapeutic response to cabazitaxel in models of advanced prostate cancer. Prostate 80:926-937 (2020). PubMed: 32542812
Jungwirth G et al. Identification of KIF11 As a Novel Target in Meningioma. Cancers (Basel) 11:N/A (2019). PubMed: 30991738

View all Publications for this product

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (14)

Customer reviews and Q&As