Anti-KIFC1 antibody [11445] (ab172620)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-KIFC1 antibody [11445]
See all KIFC1 primary antibodies -
DescriptionRabbit monoclonal [11445] to KIFC1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human KIFC1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BW19 -
Positive control
- Human tonsil tissue; Human cervical carcinoma; HeLa, HepG2 and 293T cell lysates; Immunoprecipitation pellet from 293T cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone number11445
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab172620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 74 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. |
Target
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FunctionMinus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.
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Sequence similaritiesBelongs to the kinesin-like protein family. NCD subfamily.
Contains 1 kinesin-motor domain. -
Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis.
- Information by UniProt
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Database links
- Entrez Gene: 3833 Human
- Omim: 603763 Human
- SwissProt: Q9BW19 Human
- Unigene: 436912 Human
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Alternative names
- HSET antibody
- HSET KNSL2 antibody
- KIF C1 antibody
see all
Images
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![Western blot - Anti-KIFC1 antibody [11445] (ab172620)](https://www.abcam.com/ps/products/172/ab172620/Images/ab172620-293581-Ab172620-WB-KO-HDRE.jpg)
Western blot - Anti-KIFC1 antibody [11445] (ab172620)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KIFC1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HepG2 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab172620 observed at 74 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab172620 was shown to specifically react with KIFC1 in wild-type cells as signal was lost in KIFC1 knockout cells. Wild-type and KIFC1 knockout samples were subjected to SDS-PAGE. Ab172620 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 0.228 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-KIFC1 antibody [11445] (ab172620)](https://www.abcam.com/ps/products/172/ab172620/Images/ab172620-195759-5.jpg)
Western blot - Anti-KIFC1 antibody [11445] (ab172620)All lanes : Anti-KIFC1 antibody [11445] (ab172620) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 74 kDa -
![Western blot - Anti-KIFC1 antibody [11445] (ab172620)](https://www.abcam.com/ps/products/172/ab172620/Images/ab172620-195758-4.jpg)
Western blot - Anti-KIFC1 antibody [11445] (ab172620)Western blot analysis on immunoprecipitation pellet from 293T cell lysate, labeling KIFC1 with ab172620 at 1/10 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)](https://www.abcam.com/ps/products/172/ab172620/Images/ab172620-195757-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue laneling KIFC1 with ab172620 at 1/50 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)](https://www.abcam.com/ps/products/172/ab172620/Images/ab172620-195756-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC1 antibody [11445] (ab172620)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling KIFC1 with ab172620 at 1/50 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)](https://www.abcam.com/ps/products/172/ab172620/Images/ab172620-195755-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KIFC1 antibody [11445] (ab172620)Immunofluorescent staining of HeLa cells labeling KIFC1 with ab172620 at 1/50 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- She ZY et al. Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus. Oncotarget 8:36469-36483 (2017). Read more (PubMed: 28430595) »
- Xiao YX et al. C-terminal kinesin motor KIFC1 participates in facilitating proper cell division of human seminoma. Oncotarget 8:61373-61384 (2017). WB ; Human . Read more (PubMed: 28977870) »
