Product nameAnti-KIFC1 antibody
See all KIFC1 primary antibodies
DescriptionRabbit polyclonal to KIFC1
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
Synthetic peptide corresponding to a region between residues 1 and 50 of human KIFC1 (NP_002254.1).
- Whole cell lysate from HeLa and 293T cells. This antibody gave a positive result when used in the following methanol fixed cell lines: T47D
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab72452 was affinity purified using an epitope specific to KIFC1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab72452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMinus end-directed microtubule-dependent motor required for bipolar spindle formation. May contribute to movement of early endocytic vesicles.
Sequence similaritiesBelongs to the kinesin-like protein family. NCD subfamily.
Contains 1 kinesin-motor domain.
Cellular localizationNucleus. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Early endosome. Associated with nucleus during interphase, centrosomes in early and spindle in later mitosis.
- Information by UniProt
- HSET antibody
- HSET KNSL2 antibody
- KIF C1 antibody
All lanes : Anti-KIFC1 antibody (ab72452) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 220 kDa, 300 kDa, 33 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling KIFC1 with ab72452 at 1/5000 (0.2µg/ml). Detection: DAB.
ICC/IF image of ab72452 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab72452 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
HeLa whole cell lysate, (1 mg for IP, 20% of IP loaded) treated with ab72452 at 3µg/mg lysate for IP, and at 1µg/ml for subsequent WB.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab72452 has been referenced in 1 publication.
- Li Y et al. KIFC1 is a novel potential therapeutic target for breast cancer. Cancer Biol Ther 16:1316-22 (2015). PubMed: 26177331