Anti-KIFC3 antibody (ab154419)
Key features and details
- Rabbit polyclonal to KIFC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIFC3 antibody -
Description
Rabbit polyclonal to KIFC3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 51-294 of Human KIFC3.
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Positive control
- NT2D1 and HeLa cell lysates; HeLa cells; Human colon carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 93 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Minus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance. -
Sequence similarities
Belongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
Cellular localization
Cell junction > adherens junction. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic vesicle membrane. Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts. - Information by UniProt
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Database links
- Entrez Gene: 3801 Human
- Entrez Gene: 16582 Mouse
- Omim: 604535 Human
- SwissProt: Q9BVG8 Human
- SwissProt: O35231 Mouse
- Unigene: 23131 Human
- Unigene: 378951 Mouse
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Alternative names
- DKFZp686D23201 antibody
- FLJ34694 antibody
- Kifc3 antibody
see all
Images
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Western blot - Anti-KIFC3 antibody (ab154419)Anti-KIFC3 antibody (ab154419) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KIFC3 antibody (ab154419)Immunofluorescent analysis of methanol-fixed HeLa cells labeling KIFC3 with ab154419 at 1/200 dilution. Lower panel co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC3 antibody (ab154419)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling KIFC3 with ab154419 at 1/500 dilution.
Protocols
References (0)
ab154419 has not yet been referenced specifically in any publications.
