Overview

  • Product name
  • Description
    Rabbit polyclonal to KIFC3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 51-294 of Human KIFC3.

  • Positive control
    • NT2D1 and HeLa cell lysates; HeLa cells; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Minus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance.
  • Sequence similarities
    Belongs to the kinesin-like protein family.
    Contains 1 kinesin-motor domain.
  • Cellular localization
    Cell junction > adherens junction. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic vesicle membrane. Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686D23201 antibody
    • FLJ34694 antibody
    • Kifc3 antibody
    • KIFC3_HUMAN antibody
    • Kinesin family member C3 antibody
    • Kinesin-like protein KIFC3 antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling KIFC3 with ab154419 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling KIFC3 with ab154419 at 1/500 dilution.
  • Anti-KIFC3 antibody (ab154419) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 93 kDa



    7.5% SDS PAGE

References

ab154419 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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