Anti-KIFC3 antibody (ab154419)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-KIFC3 antibody
See all KIFC3 primary antibodies -
DescriptionRabbit polyclonal to KIFC3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 51-294 of Human KIFC3.
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Positive control
- NT2D1 and HeLa cell lysates; HeLa cells; Human colon carcinoma tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 93 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). | |
| ICC/IF | 1/100 - 1/1000. |
Target
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FunctionMinus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance.
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Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain. -
Cellular localizationCell junction > adherens junction. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic vesicle membrane. Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts.
- Information by UniProt
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Database links
- Entrez Gene: 3801 Human
- Entrez Gene: 16582 Mouse
- Omim: 604535 Human
- SwissProt: Q9BVG8 Human
- SwissProt: O35231 Mouse
- Unigene: 23131 Human
- Unigene: 378951 Mouse
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Alternative names
- DKFZp686D23201 antibody
- FLJ34694 antibody
- Kifc3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-KIFC3 antibody (ab154419)Immunofluorescent analysis of methanol-fixed HeLa cells labeling KIFC3 with ab154419 at 1/200 dilution. Lower panel co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIFC3 antibody (ab154419)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling KIFC3 with ab154419 at 1/500 dilution. -
Western blot - Anti-KIFC3 antibody (ab154419)Anti-KIFC3 antibody (ab154419) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE
Protocols
References
ab154419 has not yet been referenced specifically in any publications.
