Key features and details
- Rabbit polyclonal to KIFC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KIFC3 antibody
DescriptionRabbit polyclonal to KIFC3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment, corresponding to a region within amino acids 51-294 of Human KIFC3.
- NT2D1 and HeLa cell lysates; HeLa cells; Human colon carcinoma tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 93 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionMinus-end microtubule-dependent motor protein. Involved in apically targeted transport (By similarity). Required for zonula adherens maintenance.
Sequence similaritiesBelongs to the kinesin-like protein family.
Contains 1 kinesin-motor domain.
Cellular localizationCell junction > adherens junction. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic vesicle membrane. Apical cell membrane. On membrane organelles immediately beneath the apical plasma membrane of renal tubular epithelial cells. Localized in the distal tubules and loops of Henle in the kidney, but not in the proximal tubules or the glomeruli, with stronger staining in the apical area of these epithelial cells (By similarity). Localizes along zonula adherens only at mature cell-cell contacts.
- Information by UniProt
- DKFZp686D23201 antibody
- FLJ34694 antibody
- Kifc3 antibody
Anti-KIFC3 antibody (ab154419) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 93 kDa
7.5% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells labeling KIFC3 with ab154419 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling KIFC3 with ab154419 at 1/500 dilution.
ab154419 has not yet been referenced specifically in any publications.