Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Purification notesPurified by peptide affinity chromatography. Antibody is lyophilized from PBS buffer with 2% sucrose.
Our Abpromise guarantee covers the use of ab22842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 5 µg/ml. Predicted molecular weight: 45 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
FunctionInvolved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.
Tissue specificityUbiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).
Sequence similaritiesBelongs to the KIN17 family.
Contains 1 C2H2-type zinc finger.
DomainThe C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding.
Cellular localizationNucleus. Cytoplasm. During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids.
- Information by UniProt
- antigenic determinant of recA protein homolog antibody
- Binding to curved DNA antibody
- BTCD antibody
Anti-KIN antibody (ab22842) at 1 µg/ml + HepG2 cell lysate with 5% skim milk / PBS buffer
HRP conjugated anti-Rabbit IgG diluted in 1: 50,000 - 100,025.
Performed under reducing conditions.
Predicted band size: 45 kDa
Gel concentration 12%
Paraffin embeded tissue: Human kidney, Cells with positive label (indicated with arrows): Epithelial cells of renal tubule, Antibody concentration: 4-8 µg/ml, Magnification: x400
Paraffin embeded tissue: Human spermatophore, Cells with positive label (indicated with arrows): Epithelial cells, Antibody concentration: 4-8 µg/ml, Magnification: x400
ab22842 has not yet been referenced specifically in any publications.