Product nameKinase Assay Buffer I
Stock solution of buffer used for assaying kinase protein.
Formulation: Frozen solution of buffer containing: 25 mM MOPS, pH7.2, 12.5 mM ß-glycerol-phosphate, 25 mM MgCl2, 5 mM EGTA and 2 mM EDTA. Add to final concentration of 0.25 mM DTT prior to use. DTT solution supplied in separate vial.
Sterility: Buffer passed through a 0.2µ filter membrane and packaged aseptically.
Storage and Stability: Store product at -20°C (stable for 1 yr at -20°C from date of shipment). For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab189135 has not yet been referenced specifically in any publications.