Overview

  • Product name

    Kinase Assay Buffer I
  • Product overview

    Stock solution of buffer used for assaying kinase protein.

  • Notes

    Formulation: Frozen solution of buffer containing: 25 mM MOPS, pH7.2, 12.5 mM ß-glycerol-phosphate, 25 mM MgCl2, 5 mM EGTA and 2 mM EDTA. Add to final concentration of 0.25 mM DTT prior to use. DTT solution supplied in separate vial.

     

    Sterility: Buffer passed through a 0.2µ filter membrane and packaged aseptically.

     

    Storage and Stability: Store product at -20°C (stable for 1 yr at -20°C from date of shipment). For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 ml 5 ml 20 ml
    DTT (1M) 1 x 10µl 1 x 10µl 1 x 10µl
    Kinase Assay Buffer I 1 x 1ml 1 x 5ml 1 x 20ml
  • Research areas

References

ab189135 has not yet been referenced specifically in any publications.

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