Kinase Assay Buffer I (ab189135)
Overview
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Product name
Kinase Assay Buffer I -
Product overview
Stock solution of buffer used for assaying kinase protein.
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Notes
Formulation: Frozen solution of buffer containing: 25 mM MOPS, pH7.2, 12.5 mM ß-glycerol-phosphate, 25 mM MgCl2, 5 mM EGTA and 2 mM EDTA. Add to final concentration of 0.25 mM DTT prior to use. DTT solution supplied in separate vial.
Sterility: Buffer passed through a 0.2µ filter membrane and packaged aseptically.
Storage and Stability: Store product at -20°C (stable for 1 yr at -20°C from date of shipment). For optimal storage, aliquot buffer into smaller quantities after centrifugation and store at recommended temperature. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 ml 5 ml 20 ml DTT (1M) 1 x 10µl 1 x 10µl 1 x 10µl Kinase Assay Buffer I 1 x 1ml 1 x 5ml 1 x 20ml -
Research areas
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab189135 has been referenced in 1 publication.
- Jin X et al. PES1 promotes BET inhibitors resistance and cells proliferation through increasing c-Myc expression in pancreatic cancer. J Exp Clin Cancer Res 38:463 (2019). PubMed: 31718704