Key features and details
- Rabbit polyclonal to Kindlin 2
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Kindlin 2 antibody
See all Kindlin 2 primary antibodies
DescriptionRabbit polyclonal to Kindlin 2
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide corresponding to Human Kindlin 2 aa 450-550 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab74030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).|
FunctionParticipates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
Tissue specificityUbiquitous. Found in numerous tumor tissues.
Sequence similaritiesBelongs to the kindlin family.
Contains 1 FERM domain.
Contains 1 PH domain.
DomainThe FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
Cellular localizationCytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
- Information by UniProt
- FLJ34213 antibody
- FLJ44462 antibody
- UNC112 antibody
All lanes : Anti-Kindlin 2 antibody (ab74030) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : FERMT2 knockout HeLa cell lysate
Lane 3 : Saos-2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Lanes 1-3: Merged signal (red and green). Green - ab74030 observed at 78 kDa. Red - loading control ab8245 observed at 36 kDa.
ab74030 Anti-Kindlin 2 antibody was shown to specifically react with Kindlin 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265247 (knockout cell lysate ab257436) was used. Wild-type and Kindlin 2 knockout samples were subjected to SDS-PAGE. ab74030 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml
Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
Lane 3 : Human testis tissue lysate - total protein (ab30257)
Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Exposure time: 8 minutes
Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 78kDa; Kindlin 2
The kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.
ab74030 has been referenced in 8 publications.
- Boscher E et al. Copy Number Variants in miR-138 as a Potential Risk Factor for Early-Onset Alzheimer's Disease. J Alzheimers Dis 68:1243-1255 (2019). PubMed: 30909216
- Wei CY et al. Elevated kindlin-2 promotes tumour progression and angiogenesis through the mTOR/VEGFA pathway in melanoma. Aging (Albany NY) 11:6273-6285 (2019). PubMed: 31427543
- Spiess M et al. Active and inactive ß1 integrins segregate into distinct nanoclusters in focal adhesions. J Cell Biol 217:1929-1940 (2018). PubMed: 29632027
- Crowell LE et al. On-demand manufacturing of clinical-quality biopharmaceuticals. Nat Biotechnol N/A:N/A (2018). PubMed: 30272677
- Liu S et al. Prognostic value of Kindlin-2 expression in patients with solid tumors: a meta-analysis. Cancer Cell Int 18:166 (2018). PubMed: 30386175
- Artym VV et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol 208:331-50 (2015). PubMed: 25646088
- Wu C et al. Kindlin-2 controls TGF-ß signalling and Sox9 expression to regulate chondrogenesis. Nat Commun 6:7531 (2015). IHC . PubMed: 26151572
- Steinberg F et al. SNX17 protects integrins from degradation by sorting between lysosomal and recycling pathways. J Cell Biol 197:219-30 (2012). PubMed: 22492727