Overview

  • Product name

  • Description

    Rabbit polyclonal to Kindlin 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Kindlin 2 aa 450-550 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab74029)

  • Positive control

    • This antibody gave a positive signal in the following lysates: HT 1080 Whole Cell, SAOS-2 Whole Cell, Human Testis Tissue and HeLa Nuclear

Properties

Applications

Our Abpromise guarantee covers the use of ab74030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Can be blocked with Human Kindlin 2 peptide (ab74029).
ICC/IF 1/100.

Target

  • Function

    Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
  • Tissue specificity

    Ubiquitous. Found in numerous tumor tissues.
  • Sequence similarities

    Belongs to the kindlin family.
    Contains 1 FERM domain.
    Contains 1 PH domain.
  • Domain

    The FERM domain is not correctly detected by PROSITE or Pfam techniques because it contains the insertion of a PH domain.
  • Cellular localization

    Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Within actin stress fibers at cell-ECM focal adhesion sites.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ34213 antibody
    • FLJ44462 antibody
    • UNC112 antibody
    • FERM2_HUMAN antibody
    • Fermitin family homolog 2 antibody
    • Fermt2 antibody
    • KIND2 antibody
    • Kindlin-2 antibody
    • MIG-2 antibody
    • MIG2 antibody
    • Mitogen-inducible gene 2 protein antibody
    • PH domain-containing family C member 1 antibody
    • Pleckstrin homology domain containing family C (with FERM domain) member 1 antibody
    • Pleckstrin homology domain-containing family C member 1 antibody
    • PLEKHC1 antibody
    • UNC112B antibody
    see all

Images

  • All lanes : Anti-Kindlin 2 antibody (ab74030) at 1 µg/ml

    Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 2 : SAOS-2 (sarcoma osteogenic) Whole Cell lysate
    Lane 3 : Human testis tissue lysate - total protein (ab30257)
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 78 kDa
    Observed band size: 78 kDa


    Exposure time: 8 minutes
  • Kindlin 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Kindlin 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab74030.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 78kDa; Kindlin 2
  • The kindlin 2 antibody (ab74030) was tested in lysate obtained from CHO cells either expressing endogenous levels of kindlin 2 (lane1)and transfected CHO cells in which flag-tagged kindlin2 was overexpressed (lane 2). A specific band was at the expected molecular weight of approximately 72 kDa (+/- Flag-tag). ß-actin was used to show loading in the two lanes.

    See Abreview

  • ab74030 staining Kindlin 2 in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using Triton, then incubated with ab74030 at a 1/100 dilution for 1 hour at 25°C. The secondary used was a Cy3-AffiniPure F(ab')2 fragment goat anti-rabbit monoclonal, used at a 1/200 dilution.

    See Abreview

References

This product has been referenced in:

  • Boscher E  et al. Copy Number Variants in miR-138 as a Potential Risk Factor for Early-Onset Alzheimer's Disease. J Alzheimers Dis 68:1243-1255 (2019). Read more (PubMed: 30909216) »
  • Spiess M  et al. Active and inactive ß1 integrins segregate into distinct nanoclusters in focal adhesions. J Cell Biol 217:1929-1940 (2018). Read more (PubMed: 29632027) »
See all 7 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (REF cells)
Gel Running Conditions
Reduced Denaturing (10 % SDS-PAGE)
Loading amount
20 µg
Treatment
Kindlin2 siRNA transfection for 48 hrs
Specification
REF cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 17 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Loading amount
25 µg
Specification
HEK293T cells
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 28 2013

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton

Abcam user community

Verified customer

Submitted Sep 05 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line ( cervical cancer))
Specification
HeLa cell line ( cervical cancer)
Fixative
Paraformaldehyde
Permeabilization
Yes - triton

Abcam user community

Verified customer

Submitted Sep 05 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (CHO cell)
Loading amount
40 µg
Specification
CHO cell
Treatment
+/- transfect human kindlin2
Gel Running Conditions
Reduced Denaturing (7.5)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Dr. Ana Kasirer-Friede

Verified customer

Submitted Jul 19 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Fixative
tried different conditions
Permeabilization
Yes - 0.5% tx-100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Oct 23 2009

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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