Overview

  • Product name

  • Description

    Rabbit polyclonal to Kinectin 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human Kinectin 1 aa 150-200. The exact sequence is proprietary. NP_001072989.1
    Sequence:

    K QPTPPSEAAA SKKKPGQKKS KNGSDDQDKK VETLMVPSKR QEALPLHQET


    Database link: Q86UP2

  • Positive control

    • Hela, 293T, Jurkat cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab190271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 156 kDa.

Target

  • Function

    Receptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.
  • Tissue specificity

    High levels in peripheral blood lymphocytes, testis and ovary, lower levels in spleen, thymus, prostate, small intestine and colon.
  • Sequence similarities

    Belongs to the kinectin family.
  • Cellular localization

    Endoplasmic reticulum membrane. Vesicle membrane protein anchored to the endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • CG-1 antigen antibody
    • CG1 antibody
    • CG1 antigen antibody
    • KIAA0004 antibody
    • Kinectin antibody
    • Kinesin receptor antibody
    • Ktn1 antibody
    • KTN1_HUMAN antibody
    • MU-RMS-40.19 antibody
    see all

Images

  • All lanes : Anti-Kinectin 1 antibody (ab190271) at 0.1 µg/ml

    Lane 1 : Hela whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 156 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation of 293T whole cell lysate (0.5-1 mg per IP reaction; 20% of IP loaded) labeling Kinectin 1 with ab190271 at 1 µg/ml in Western Blot.  Samples were immunoprepitated with a control antibody (1)  ab190271 at 6 µg per reaction (2) or control IgG (3).

References

ab190271 has not yet been referenced specifically in any publications.

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