Key features and details
- Rabbit polyclonal to Kinectin 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Kinectin 1 antibody
See all Kinectin 1 primary antibodies
DescriptionRabbit polyclonal to Kinectin 1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: RT4 cell lysate. ICC/IF: U-251 MG cells. IHC-P: Human testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 156 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionReceptor for kinesin thus involved in kinesin-driven vesicle motility. Accumulates in integrin-based adhesion complexes (IAC) upon integrin aggregation by fibronectin.
Tissue specificityHigh levels in peripheral blood lymphocytes, testis and ovary, lower levels in spleen, thymus, prostate, small intestine and colon.
Sequence similaritiesBelongs to the kinectin family.
Cellular localizationEndoplasmic reticulum membrane. Vesicle membrane protein anchored to the endoplasmic reticulum.
- Information by UniProt
- CG-1 antigen antibody
- CG1 antibody
- CG1 antigen antibody
Anti-Kinectin 1 antibody (ab224324) at 1/100 dilution + RT4 (human urinary bladder cancer cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 156 kDa
PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Kinectin 1 (green) using ab224324 at 4 µg/ml in ICC/IF.
Paraffin-embedded human testis tissue stained for Kinectin 1 using ab224324 at 1/50 dilution in immunohistochemical analysis.
ab224324 has not yet been referenced specifically in any publications.