Overview

  • Product name
  • Description
    Rabbit polyclonal to Kinesin 5C
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for kinesin 5C and does not detect other kinesin isotypes.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse, Cow
  • Immunogen

    Synthetic peptide corresponding to Human Kinesin 5C aa 938-957.
    Sequence:

    AVHAIRGGGGSSSNSTHYQK


    (Peptide available as ab41784)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure including a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. There are currently three known Kinesin 5 family members denoted as A, B, and C. Kinesin 5A and kinesin 5C appear to be exclusively neuronal, whereas kinesin 5B appears to be ubiquitous in its expression.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18334549
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).Can be blocked with Human Kinesin 5C peptide (ab41784).
IP 1/4000.

Target

  • Function
    Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport.
  • Tissue specificity
    Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.
  • Sequence similarities
    Belongs to the kinesin-like protein family. Kinesin subfamily.
    Contains 1 kinesin-motor domain.
  • Domain
    Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDCBM2 antibody
    • FLJ44735 antibody
    • KIAA0531 antibody
    • KIF 5C antibody
    • Kif5c antibody
    • KIF5C protein antibody
    • KIF5C_HUMAN antibody
    • Kinesin family member 5C antibody
    • Kinesin heavy chain isoform 5C antibody
    • Kinesin heavy chain neuron specific 2 antibody
    • Kinesin heavy chain neuron specific antibody
    • Kinesin heavy chain neuron-specific 2 antibody
    • Kinesin5C antibody
    • KINN antibody
    • MGC111478 antibody
    • NKHC 2 antibody
    • NKHC antibody
    • NKHC-2 antibody
    • NKHC2 antibody
    see all

Images

  • Shows a Western blot of kinesin 5C on human retinal extract using ab5630. The expected band at 110 kDa is seen along with an unidentified band at 100 kDa.

  • ab5630 staining Kinesin 5C in COS-7 cells by Immunocytochemistry/ Immunofluorescence.
    GFP-Kif5c behaviour in living cells. Cropped still images from a time-lapse recording of GFP-Kif5c puncta (green) moving along a MT labelled with mCherry–-tubulin (red) in a live COS-7 cell. The GFP-Kif5c puncta move from right to left. Scale bar, 2 µm. The graph shows the respective velocity of the GFP-Kif5c puncta seen over time, demonstrating the rapid changes in velocity of those puncta.
  • IHC image of ab5630 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5630, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Leiva-Rodríguez T  et al. ATG5 overexpression is neuroprotective and attenuates cytoskeletal and vesicle-trafficking alterations in axotomized motoneurons. Cell Death Dis 9:626 (2018). Read more (PubMed: 29799519) »
  • Malikov V & Naghavi MH Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating. Cell Rep 20:2792-2799 (2017). Read more (PubMed: 28930676) »
See all 6 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Question

Dear Abcam technical team, I want to order proteins from your website, but there are some questions before order. (1) I want to experiment with conventional kinesin heavy chain, KIF5. But there are three different members of kinesin family 5. KIF5A, KIF5B and KIF5C. I know the function of each member, A and C involves in neuronal transport otherwise B involves in inner cell transport. But is there any structural difference between three members? If is, I have to choose specific member to experiment. (2) There are two types in KIF5A and three types in KIF5B. It seems that each has different domain, so I wonder there are functional or structural difference between types. Also, your company has two different types of KIF5C. One is just (938-957) domain and the other is His-tagged protein. His tagged kinesin has higher protein purity. Is there any reason to choose His tagged KIF5C except higher purity? (3) Is antibody that has specificity of my protein (e.g. KIF5A --> Anti-kinesin 5A antibody) attach to specific domain (like C-terminal) or it is just randomly attached? For my experiment, it is good for antibody to attach to C-terminal. In other words, is antibody has C-terminal specificity? (4) Do you sell biotinylated antibody for kinesin? I want antibody that has biotin coating but your website has no option or products for biotin coating. If you cannot understand my questions, please let me know which parts you cannot understand. Then I will send the more specific explanation for my questions. From

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Answer

Thank you for contacting us and your interest in our products. 1.) Indeed, there is structural differences between those members. The sequences of the three members are not the same. I have made an alignment where you can see these differences. Please refer to the following link to see these differences: http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111011-085702-0894-35538860-oy To check out further parameters of these proteins, I can recommend to browse either the expasy site (http://web.expasy.org/ or http://web.expasy.org/protparam/) as well as one of the Bioinformatics institutes homepages, for example the EBI, http://www.ebi.ac.uk/. There are different Tools to predict the protein structures. 2.) We do have currently only one KIF5 protein in our catalogue, and this is the ab91695 Kinesin 5C protein (Tagged-His Tag) which is not a full length protein but a recombinant fragment corresponding to amino acids 500-957 of Human Kinesin 5C. Click here (or use the following: https://www.abcam.com/index.html?datasheet=91695). All the other products are indeed peptides and do correspond to the short amino acid sequence of the numbers in brackets. These peptides are too short to be able to have a function similar to the whole protein, however they can be used for blocking studies with the corresponding antibody. For example ab41784 is a peptide corresponding the sequence 938 to 957 of human KIF5C. Similarly for the other products: KIF5A: - peptide amino acid 1019-1032 is ab23133 and - peptide amino acid 1007-1027 is ab41781 KIF5B - peptide amino acid 376-396 is ab41782 - peptide amino acid 951-963 is ab23262 - peptide ab100817, a few amino acids derived from the sequence between amino acid 350 to 450 3.) Each antibody is different. I have therefore put together the following list: KIF5A, we do have two antibodies: -ab5628 is a polyclonal antibody directed against the C terminus of KIF5A, recognizing the sequences of amino acids 1007 to 1027 of KIF5A. This antibody has also been tested against the KIF5A protein of different species (human, mouse, rat and cow) as well as in immunocytochemistry, immunoprecipitation and Western blot. Click here (or use the following: https://www.abcam.com/index.html?datasheet=5628). -ab5958 which is a polyclonal antibody directed against the C terminal sequence (amino acids 1019 - 1032 of KIF5A). This antibody has however not been well characterized as yet, as you see it is one of our Fasttrack antibodies. Please let me know if you would like to know more about this. Click here (or use the following: https://www.abcam.com/index.html?datasheet=5958). KIF5B we have 7 antibodies currently: two of them are against a region in the C terminal: ab15705, corresponding to amino acids 951-963 Click here (or use the following: https://www.abcam.com/index.html?datasheet=15705). ab97762, corresponding to amino acids 901 - 963 Click here (or use the following: https://www.abcam.com/index.html?datasheet=97762). The other KIF5B antibodies are either N terminal (ab42492, immunogen amino acids 7-56), from the central region (ab5629, immunogen amino acids 376-396 and ab91033 region derived from amino acids 350 - 450) or are mouse monoclonal antibodies for which we do not know the epitope (ab110155 and ab11262) KIF5C we only have one antibody: ab5630 which has been raised against a C terminal sequence as well, the amino acids 938 - 957 of the human KIF5C. Click here (or use the following: https://www.abcam.com/index.html?datasheet=5630). 4.) Indeed we do currently not have one of these antibodies in biotinylated form. We do have however the Easylink conjugation kits, which are especially designed for easy conjugation of antibodies. We do have such kits also to perform biotin conjugation. You can find more information in this regards under the following website: www.abcam.com/easylink Again, please let me know if you wish more information about this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human SH-SY5Y neuroblastoma cells)
Loading amount
10 µg
Specification
Human SH-SY5Y neuroblastoma cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Mrs. Adelheid Hanes

Verified customer

Submitted Nov 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (forebrain)
Specification
forebrain
Immuno-precipitation step
Protein A

Dr. Kieran Brickley

Verified customer

Submitted May 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Recombinant protein (HEK 293 cells)
Loading amount
50 µg
Specification
HEK 293 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 27 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Loading amount
100 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Apr 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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