Validated using a knockout cell line

Recombinant Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)


  • Product name

    Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)]
    See all Kinesin Heavy Chain 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Kinesin Heavy Chain 2 (Uniprot ID O00139).

  • Positive control

    • Human fetal brain tissue lysate; Jurkat, 293T cell lysate; Human thyroid carcinoma tissue; Jurkat cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab154868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 187 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Relevance

      Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure which includes a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. An expansive phylogenetic tree of kinesins and kinesin-related proteins (KRPs) has been assembled based on this information.
    • Cellular localization

      Cytoplasm, cytoskeleton
    • Database links

    • Alternative names

      • HK2 antibody
      • KIF2 antibody
      • Kinesin heavy chain member 2 antibody
      • KNS2 antibody


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Kinesin Heavy Chain 2 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab154868 observed at 100 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab154868 was shown to recognize Kinesin Heavy Chain 2 in wild-type HAP1 cells as signal was lost at the expected MW in Kinesin Heavy Chain 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Kinesin Heavy Chain 2 knockout samples were subjected to SDS-PAGE. Ab154868 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescent analysis of Jurkat cells labeling Kinesin Heavy Chain 2 with ab154868 at 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma labeling Kinesin Heavy Chain 2 with ab154868 at 1/50.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • All lanes : Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 187 kDa


    ab154868 has not yet been referenced specifically in any publications.

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