Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)]
    See all Kinesin Heavy Chain 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human Kinesin Heavy Chain 2 (Uniprot ID O00139).

  • Positive control

    • Human fetal brain tissue lysate; Jurkat, 293T cell lysate; Human thyroid carcinoma tissue; Jurkat cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10740(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 187 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Relevance

      Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure which includes a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. An expansive phylogenetic tree of kinesins and kinesin-related proteins (KRPs) has been assembled based on this information.

    • Cellular localization

      Cytoplasm, cytoskeleton

    • Database links

      • Alternative names

        • HK2 antibody
        • KIF2 antibody
        • Kinesin heavy chain member 2 antibody
        • KNS2 antibody

      Images

      • Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
        Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

        Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
        Lane 2: Kinesin Heavy Chain 2 knockout HAP1 whole cell lysate (20 µg)
        Lane 3: HeLa whole cell lysate (20 µg)

        Lanes 1 - 3: Merged signal (red and green). Green - ab154868 observed at 100 kDa. Red - loading control, ab18058, observed at 130 kDa.

        ab154868 was shown to recognize Kinesin Heavy Chain 2 in wild-type HAP1 cells as signal was lost at the expected MW in Kinesin Heavy Chain 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Kinesin Heavy Chain 2 knockout samples were subjected to SDS-PAGE. Ab154868 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

      • Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
        Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
        Immunofluorescent analysis of Jurkat cells labeling Kinesin Heavy Chain 2 with ab154868 at 1/100.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

        Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma labeling Kinesin Heavy Chain 2 with ab154868 at 1/50.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
        Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
        All lanes : Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868) at 1/1000 dilution

        Lane 1 : Human fetal brain tissue lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 187 kDa

      • Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
        Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)

      References (0)

      Publishing research using ab154868? Please let us know so that we can cite the reference in this datasheet.

      ab154868 has not yet been referenced specifically in any publications.

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