Recombinant Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
![Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)](https://www.abcam.com/ps/products/154/ab154868/Images/ab154868-296555-ab154868gdbKOWB.jpg "Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)]
See all Kinesin Heavy Chain 2 primary antibodies -
Description
Rabbit monoclonal [EPR10740(B)] to Kinesin Heavy Chain 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain tissue lysate; Jurkat, 293T cell lysate; Human thyroid carcinoma tissue; Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10740(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 187 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 187 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Relevance
Kinesins are a superfamily of microtubule-associated motor proteins involved in a variety of cellular processes including membranous organelle transport and cell division. Kinesin has been found in a variety of organisms and cell types and is subject to spatial and temporal regulation. These proteins have a modular structure which includes a conserved motor domain of approximately 350 amino acids, which is responsible for microtubule binding and ATP hydrolysis. In addition to the motor domain, subfamily members share common domain organization, exhibit sequence similarity, motility properties, and cellular functions outside of the motor domain. An expansive phylogenetic tree of kinesins and kinesin-related proteins (KRPs) has been assembled based on this information. -
Cellular localization
Cytoplasm, cytoskeleton -
Database links
- Entrez Gene: 3796 Human
- Omim: 602591 Human
- SwissProt: O00139 Human
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Alternative names
- HK2 antibody
- KIF2 antibody
- Kinesin heavy chain member 2 antibody
- KNS2 antibody
Images
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Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Kinesin Heavy Chain 2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab154868 observed at 100 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab154868 was shown to recognize Kinesin Heavy Chain 2 in wild-type HAP1 cells as signal was lost at the expected MW in Kinesin Heavy Chain 2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Kinesin Heavy Chain 2 knockout samples were subjected to SDS-PAGE. Ab154868 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)](https://www.abcam.com/ps/products/154/ab154868/Images/Kinesin-Heavy-Chain-2-Primary-antibodies-ab154868-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)Immunofluorescent analysis of Jurkat cells labeling Kinesin Heavy Chain 2 with ab154868 at 1/100. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)](https://www.abcam.com/ps/products/154/ab154868/Images/Kinesin-Heavy-Chain-2-Primary-antibodies-ab154868-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma labeling Kinesin Heavy Chain 2 with ab154868 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)](https://www.abcam.com/ps/products/154/ab154868/Images/Kinesin-Heavy-Chain-2-Primary-antibodies-ab154868-3.jpg)
Western blot - Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)All lanes : Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 187 kDa -
![Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)](https://www.abcam.com/ps/products/154/ab154868/Images/ab154868-5-benefits-of-recombinant-antibodies.png)
Anti-Kinesin Heavy Chain 2 antibody [EPR10740(B)] (ab154868)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab154868 has not yet been referenced specifically in any publications.