The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at 2-5 µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 250 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The kinetochore is the protein structure in eukaryotes which assembles on the centromere and links the chromosome to microtubule polymers from the mitotic spindle during mitosis and meiosis. The kinetochore contains two regions: an inner kinetochore, which is tightly associated with the centromere DNA; and an outer kinetochore, which interacts with microtubules. Even the simplest kinetochores consist of more than 45 different proteins, many of which are conserved throughout eukaryote species.
Detection of Kinetochore by Western Blot of Immunprecipitate.
ab85996, at 1 µg/ml, staining Kinetochore in HeLa whole cell lysate immunoprecipitated using ab8599 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.