RecombinantRabMAb

Recombinant Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)

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Western blot - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • ELISA - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
  • Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6097] to Kininogen 1 - BSA and Azide free
  • Suitable for: ELISA, WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free
    See all Kininogen 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6097] to Kininogen 1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody detects three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa).    

  • Tested applications

    Suitable for: ELISA, WB, IP, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Kininogen 1 aa 600-700. The exact sequence is proprietary.

  • Positive control

    • Human plasma, platelet and ovarian cancer lysates; Human kidney tissue, Human ovarian adenocarcinoma tissue.

  • General notes

    ab226087 is the carrier-free version of ab124737 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab226087 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 2.70 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6097

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab226087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 45, 56, 120 kDa (predicted molecular weight: 48, 72 kDa).
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For antigen retrieval, heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.

Target

  • Function

    (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

  • Tissue specificity

    Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.

  • Involvement in disease

    Defects in KNG1 are the cause of high molecular weight kininogen deficiency (HMWK deficiency) [MIM:228960]. HMWK deficiency is an autosomal recessive coagulation defect. Patients with HWMK deficiency do not have a hemorrhagic tendency, but they exhibit abnormal surface-mediated activation of fibrinolysis.

  • Sequence similarities

    Contains 3 cystatin domains.

  • Post-translational
    modifications

    Bradykinin is released from kininogen by plasma kallikrein.
    Hydroxylation of Pro-383 occurs prior to the release of bradykinin.
    Phosphorylation sites are present in the extracelllular medium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • Alpha-2-thiol proteinase inhibitor antibody
      • BDK antibody
      • BK antibody
      • Bradykinin antibody
      • Bradykinin included antibody
      • Fitzgerald factor antibody
      • FLAUJEAC FACTOR antibody
      • High molecular weight kininogen antibody
      • HMWK antibody
      • Ile-Ser-Bradykinin antibody
      • Kallidin I antibody
      • Kallidin II antibody
      • KNG antibody
      • KNG1 antibody
      • KNG1_HUMAN antibody
      • Low molecular weight growth-promoting factor antibody
      • WILLIAMS FACTOR antibody
      • Williams-Fitzgerald-Flaujeac factor antibody
      see all

    Images

    • Western blot - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Western blot - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      All lanes : Anti-Kininogen 1 antibody [EPR6097] (ab124737) at 1/10000 dilution

      Lane 1 : Human plasma
      Lane 2 : Human platelet tissue lysate
      Lane 3 : Human ovarian cancer tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 48, 72 kDa



      This data was developed using ab124737, the same antibody clone in a different buffer formulation.

      ab124737 is detecting three isoforms of Kininogen 1: full length (120 kDa), light chain (56 kDa), and cleavage form (45 kDa). 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      This data was developed using ab124737, the same antibody clone in a different buffer formulation.ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human kidney tissues by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      This data was developed using ab124737, the same antibody clone in a different buffer formulation.ab124737 at 1/100 dilution staining Kininogen 1 in paraffin-embedded Human ovarian adenocarcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • ELISA - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      ELISA - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)

      This data was developed using ab124737, the same antibody clone in a different buffer formulation.

      ELISA analysis of Human Kininogen-1 recombinant protein at 1000 ng/mL with ab124737. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      OI-RD Scanning - Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      This data was developed using ab124737, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)
      Anti-Kininogen 1 antibody [EPR6097] - BSA and Azide free (ab226087)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226087? Please let us know so that we can cite the reference in this datasheet.

    ab226087 has not yet been referenced specifically in any publications.

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