Anti-Kir2.1 antibody [EPR4530] (ab109750)


  • Product name
    Anti-Kir2.1 antibody [EPR4530]
    See all Kir2.1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4530] to Kir2.1
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Kir2.1 aa 100-200 (extracellular). The exact sequence is proprietary.

  • Positive control
    • 293T and A549 cell lysates; Human brain tissue; SH SY5Y cells.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab109750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
    • Tissue specificity
      Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
    • Involvement in disease
      Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
      Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
    • Sequence similarities
      Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Cardiac inward rectifier potassium channel antibody
      • HHBIRK 1 antibody
      • HHBIRK1 antibody
      • HHIRK 1 antibody
      • HHIRK1 antibody
      • HIRK 1 antibody
      • hIRK1 antibody
      • Inward rectifier K antibody
      • Inward rectifier K(+) channel Kir2.1 antibody
      • Inward rectifier potassium channel 2 antibody
      • inwardly rectifying subfamily J member 2 antibody
      • IRK 1 antibody
      • IRK-1 antibody
      • IRK1 antibody
      • IRK2_HUMAN antibody
      • KCNJ2 antibody
      • KIR2.1 antibody
      • LQT 7 antibody
      • LQT7 antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 2 antibody
      • Potassium inwardly rectifying channel J2 antibody
      • Potassium inwardly rectifying channel subfamily J member 2 antibody
      • SQT 3 antibody
      • SQT3 antibody
      see all


    • All lanes : Anti-Kir2.1 antibody [EPR4530] (ab109750) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 48 kDa

    • ab109750 at 1/250 dilution staining Kir2.1 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • ab109750 at 1/100 dilution staining Kir2.1 in SH SY5Y cells by Immunofluorescence.


    This product has been referenced in:
    • Fancher IS  et al. Hypercholesterolemia-Induced Loss of Flow-Induced Vasodilation and Lesion Formation in Apolipoprotein E-Deficient Mice Critically Depend on Inwardly Rectifying K+ Channels. J Am Heart Assoc 7:N/A (2018). WB ; Mouse . Read more (PubMed: 29502106) »
    • Utrilla RG  et al. Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone. Front Physiol 8:903 (2017). Read more (PubMed: 29184507) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thank you for your message.

    I am sorry to confirm that becuase we have over 70 000 products, it is not feasable for us to keep small sample vials to send to customers.

    I would like to reassure you that ab65796 and ab80969 have bee...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Yes - 0.05% Triton X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted May 10 2013

    Thank youand your customer very much for your interest in our antibodies.

    I checked all immunogens and homology to zebrafish (sequnec that your customer provided)of the Kir2.1 antibodies we offer at the moment.

    Only one antibody sho...

    Read More


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