Overview

  • Product name

  • Description

    Rabbit polyclonal to Kir2.1/BIK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Kir2.1/BIK (N terminal).

  • General notes

     This product was previously labelled as Kir2.1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab65796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 48 kDa.
IHC-P 1/1000.
ICC/IF 1/1000.

Target

  • Function

    Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.
  • Tissue specificity

    Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.
  • Involvement in disease

    Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
    Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.
  • Sequence similarities

    Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cardiac inward rectifier potassium channel antibody
    • HHBIRK 1 antibody
    • HHBIRK1 antibody
    • HHIRK 1 antibody
    • HHIRK1 antibody
    • HIRK 1 antibody
    • hIRK1 antibody
    • Inward rectifier K antibody
    • Inward rectifier K(+) channel Kir2.1 antibody
    • Inward rectifier potassium channel 2 antibody
    • inwardly rectifying subfamily J member 2 antibody
    • IRK 1 antibody
    • IRK-1 antibody
    • IRK1 antibody
    • IRK2_HUMAN antibody
    • KCNJ2 antibody
    • KIR2.1 antibody
    • LQT 7 antibody
    • LQT7 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 2 antibody
    • Potassium inwardly rectifying channel J2 antibody
    • Potassium inwardly rectifying channel subfamily J member 2 antibody
    • SQT 3 antibody
    • SQT3 antibody
    see all

Images

  • ICC/IF image of ab65796 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65796, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab65796 staining Kir2.1/BIK in human colon.
    Left panel: with primary antibody at 1:1000 dilution. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References

This product has been referenced in:

  • Sun C  et al. Association study between inwardly rectifying potassium channels 2.1 and 4.1 and autism spectrum disorders. Life Sci 213:183-189 (2018). Read more (PubMed: 30304693) »
  • Utrilla RG  et al. Kir2.1-Nav1.5 Channel Complexes Are Differently Regulated than Kir2.1 and Nav1.5 Channels Alone. Front Physiol 8:903 (2017). Read more (PubMed: 29184507) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for your message.

I am sorry to confirm that becuase we have over 70 000 products, it is not feasable for us to keep small sample vials to send to customers.

I would like to reassure you that ab65796 and ab80969 have been tested in western blot, and we provide our 6 month guarantee for this application. However, as they have been tested externally, we do not currently have a WB image for them.

Therefore, I can offer a discount off a future purchase if you buy either ab65796 or ab80969 now, use it WB and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed with this offer, and which of these two antibody you woudl like to try. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase ab65796 or ab80969 either by phone, fax, or online (www.abcam.com).

3. Use it in WB.

4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

With regards to ab109750, I can confirm that 293T cells express Kir2.1 endogenously, and no treatment is needed. Other suppliers also used 293T as a positive control with Kir2.1 antibodies.

The following paper describes Kir2.1 endogenously expressed in 293T cells:
http://jp.physoc.org/content/563/3/725.full.pdf

I hope this will be helpful to you. If you have any further questions, or if you would like to accept the testing offer, please do not hesitate to contact me.

Read More

Answer

Thank you for contacting us.
The immunogen of ab80969 shares 85% homology with Zebrafish so it is more likely to cross-react. Once you decide to buy the antibody I would like to inform you about the Abreview 100% discount programme. Please click the link
https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
We are also giving away free protein ladder with primary orders. Please check
https://www.abcam.com/index.html?pageconfig=resource&rid=14968
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Question
Answer

Thank youand your customer very much for your interest in our antibodies.

I checked all immunogens and homology to zebrafish (sequnec that your customer provided)of the Kir2.1 antibodies we offer at the moment.

Only one antibody shows a sufficiently high homology to expect cross-reactivity: ab71574



Therefore, I can offer a discount off a future purchase if your customerbuys ab71574 now, tests it inzebrafish and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer'sfeedback. The discount would be to the value of ab71574 and can be used to order any product.

If your customer is interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that your customer ( please include theire-mail address)would like to proceed and test ab71574 in zebrafish. I will then send a discount code. This code must be issued before purchasing ab71574 so please wait for my reply before ordering.

2. Purchase ab71574 either by phone, fax, or online (www.abcam.com).

3. Test it in zebrafish.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab71574turns out to be unsuitable for zebrafish, your customerwill still receive the discount ontheir next purchase after your Abreview has been submitted.

Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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