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Many thanks for your reply. Would it be possible for you to send me some samples of these Abs to test? There are no pictures of westerns on your website for ab65796 and ab80969. I can send you pics of any westerns we do with them.
For the western with ab109750 on your website, were the 293T cells transfected with Kir2.1? I don't think 293T cells express Kir2.1 endogenously.
Also, if you could possibly find out whether HeLa cells need to be treated with staurosporine to get a good Kir2.1 signal, that would be very useful info.
Asked on Mar 02 2012
Thank you for your message.
I am sorry to confirm that becuase we have over 70 000 products, it is not feasable for us to keep small sample vials to send to customers.
I would like to reassure you that ab65796 and ab80969 have been tested in western blot, and we provide our 6 month guarantee for this application. However, as they have been tested externally, we do not currently have a WB image for them.
Therefore, I can offer a discount off a future purchase if you buy either ab65796 or ab80969 now, use it WB and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.
It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed with this offer, and which of these two antibody you woudl like to try. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.
2. Purchase ab65796 or ab80969 either by phone, fax, or online (www.abcam.com).
3. Use it in WB.
4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated!
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.
Please let me know if you have any questions about this offer and I would be happy to help you further.
With regards to ab109750, I can confirm that 293T cells express Kir2.1 endogenously, and no treatment is needed. Other suppliers also used 293T as a positive control with Kir2.1 antibodies.
The following paper describes Kir2.1 endogenously expressed in 293T cells:
I hope this will be helpful to you. If you have any further questions, or if you would like to accept the testing offer, please do not hesitate to contact me.
Answered on Mar 02 2012