Recombinant Anti-Kir2.1/KCNJ2 antibody [EPR4530] (ab109750)

Thank you for your message.

I am sorry to confirm that becuase we have over 70 000 products, it is not feasable for us to keep small sample vials to send to customers.

I would like to reassure you that ab65796 and ab80969 have been tested in western blot, and we provide our 6 month guarantee for this application. However, as they have been tested externally, we do not currently have a WB image for them.

Therefore, I can offer a discount off a future purchase if you buy either ab65796 or ab80969 now, use it WB and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody.

It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed with this offer, and which of these two antibody you woudl like to try. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase ab65796 or ab80969 either by phone, fax, or online (www.abcam.com).

3. Use it in WB.

4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated!

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

With regards to ab109750, I can confirm that 293T cells express Kir2.1 endogenously, and no treatment is needed. Other suppliers also used 293T as a positive control with Kir2.1 antibodies.

The following paper describes Kir2.1 endogenously expressed in 293T cells:
http://jp.physoc.org/content/563/3/725.full.pdf

I hope this will be helpful to you. If you have any further questions, or if you would like to accept the testing offer, please do not hesitate to contact me.

Thank youand your customer very much for your interest in our antibodies.

I checked all immunogens and homology to zebrafish (sequnec that your customer provided)of the Kir2.1 antibodies we offer at the moment.

Only one antibody shows a sufficiently high homology to expect cross-reactivity: ab71574



Therefore, I can offer a discount off a future purchase if your customerbuys ab71574 now, tests it inzebrafish and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer'sfeedback. The discount would be to the value of ab71574 and can be used to order any product.

If your customer is interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that your customer ( please include theire-mail address)would like to proceed and test ab71574 in zebrafish. I will then send a discount code. This code must be issued before purchasing ab71574 so please wait for my reply before ordering.

2. Purchase ab71574 either by phone, fax, or online (www.abcam.com).

3. Test it in zebrafish.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab71574turns out to be unsuitable for zebrafish, your customerwill still receive the discount ontheir next purchase after your Abreview has been submitted.

Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.