Product nameAnti-KIR2.3/HIR antibody
See all KIR2.3/HIR primary antibodies
DescriptionRabbit polyclonal to KIR2.3/HIR
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- WB: A549, HeLa, HEK-293T, HepG2, Jurkat and MCF7 whole cell lysates; Rat heart lysate. IHC-P: Human skeletal muscle and heart tissues.
Previously labelled as KIR2.3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- MCF7 whole cell lysate (ab29537)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- MCF7 whole cell lysate (ab3871)
- Jurkat whole cell lysate (ab7899)
- HepG2 whole cell lysate (ab7900)
- A549 whole cell lysate (ab7910)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab230214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 50 kDa.|
FunctionThis receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
Tissue specificityHeart, skeletal muscle, and several different brain regions including the hippocampus.
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ4 subfamily.
DomainThe Val/Gly/Ala/Pro stretch may have a functional role in the conductance or permeation properties.
Cellular localizationCell membrane. Cytoplasmic vesicle membrane. TAX1BP3 binding promotes dissociation of KCNJ4 from LIN7 famaly members and KCNJ4 internalization.
- Information by UniProt
- Hippocampal inward rectifier antibody
- Hippocampal inward rectifier potassium channel antibody
- HIR antibody
All lanes : Anti-KIR2.3/HIR antibody (ab230214) at 1/1000 dilution
Lane 1 : Rat heart tissue lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraffin-embedded human skeletal muscle tissue stained for KIR2.3/HIR using ab230214 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human heart tissue stained for KIR2.3/HIR using ab230214 at 1/100 dilution in immunohistochemical analysis.
ab230214 has not yet been referenced specifically in any publications.